Human Keratin, type I cytoskeletal 18 fragment (K18-Asp396）ELISA Kit

產(chǎn)品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人細胞角蛋白18片段Asp396(K18-Asp396)酶聯(lián)免疫試劑盒（CSB-E13696h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物.樣本中的CK 18M30含量。試劑盒檢測范圍為15.6 mIU/ml-1000 mIU/ml，適用于體外研究細胞凋亡信號通路、評估藥物肝毒性引起的上皮細胞損傷、探索炎癥性疾病中組織修復(fù)機制等科研實驗本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

CK 18M30
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates.
檢測范圍：

15.6 mIU/ml-1000 mIU/ml
靈敏度：

3.9 mIU/ml
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CK 18-M30 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
Range %	85-94
1:2	Average %	86
Range %	81-92
1:4	Average %	94
Range %	90-99
1:8	Average %	89
Range %	83-96

回收率：

The recovery of human CK 18-M30 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-100
EDTA plasma (n=4)	99	94-105

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.