Human Interferon kappa(IFNK) ELISA kit

說明書下載

中文名稱：

人干擾素κ(IFNK)酶聯免疫試劑盒
貨號：

CSB-EL011053HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人干擾素κ(IFNK)酶聯免疫試劑盒（CSB-EL011053HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFNK含量。IFNK即干擾素κ，是I型干擾素家族成員。它具有抗病毒、抗腫瘤和免疫調節等潛在作用。其研究機制方面，能與細胞表面受體結合，激活相關信號通路，誘導多種抗病毒蛋白產生，發揮免疫調節活性，抵御病原體入侵與腫瘤發展。試劑盒檢測范圍為15.6 pg/mL-1000 pg/mL，適用于病毒感染機制、自身免疫疾病模型構建、皮膚屏障功能研究等科研領域，還特別適用于免疫微環境分析、細胞因子網絡研究等基礎科研場景，能為探索IFNK在炎癥性疾病、腫瘤免疫調控及組織特異性免疫應答中的作用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFN kappa ELISA Kit; IFN-kappa ELISA Kit; Ifnk ELISA Kit; IFNK_HUMAN ELISA Kit; Interferon kappa ELISA Kit
縮寫：

IFNK
Uniprot No.：

Q9P0W0
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

15.6 pg/mL-1000 pg/mL
靈敏度：

3.9 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human IFNK in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	85
	Range %	80-90
1:4	Average %	90
	Range %	85-94
1:8	Average %	98
	Range %	94-103

回收率：

The recovery of human IFNK spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	101	97-105
EDTA plasma (n=4)	92	88-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1000	2.694	2.758	2.726	2.622
500	2.309	2.182	2.246	2.142
250	1.515	1.601	1.558	1.454
125	0.954	1.004	0.979	0.875
62.5	0.521	0.560	0.541	0.437
31.25	0.287	0.293	0.290	0.186
15.6	0.193	0.205	0.199	0.095
0	0.100	0.107	0.104

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

May play a role in the regulation of immune cell function. Cytokine that imparts cellular protection against viral infection in a species-specific manner. Activates the interferon-stimulated response element signaling pathway. It is able to directly modulate cytokine release from monocytes and dendritic cells. Binds heparin.
基因功能參考文獻：
1. investigation of common variable immunodeficiency (CVID) from 2 German families; report on the occurrence of a common and one novel truncating IFNK mutation in cases with CVID; the frequency distribution of c.30_31insTGTT in cases and controls as well as the observed segregation patterns in CVID families exclude IFNK mutations as major risk factor in CVID PMID: 28324805
2. These results suggest that high-risk human papillomavirus 31 target interferon kappa to prevent Sp100 expression and identify Sp100 as an interferon-stimulated gene with anti-human papillomavirus activity. PMID: 26491169
3. This study demonstrates that E2 proteins of high risk human papillomavirus reduce STING and IFN-kappa transcription. PMID: 24614210
4. The viral E6 and E7 oncogenes are sufficient for interferon-kappa repression, with E6 being mainly responsible. PMID: 21849431
5. IFN-kappa is down-regulated in cervical keratinocytes harboring HPV, which may be a contributing factor in the progression of a cervical lesion PMID: 20479716
6. Studied IFNK single nucleotide polymorphisms in 3982 Systemic lupus erythematosus cases and 4275 controls. PMID: 20706608
7. IFN-kappa is able to directly modulate cytokine release from monocytes and dendritic cells, inhibit inducible IL-12 release from monocytes, and bind strongly to heparin. PMID: 12391192
8. This is the first report showing an epigenetic silencing of type I IFN after HPV16 oncogene expression PMID: 19887612
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亞細胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
組織特異性：

Expressed in keratinocytes, monocytes and in resting dendritic cells.
數據庫鏈接：

HGNC: 21714

OMIM: 615326

KEGG: hsa:56832

STRING: 9606.ENSP00000276943

UniGene: Hs.591083

Human Interferon kappa(IFNK) ELISA kit

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