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中文名稱:人免疫球蛋白D(IgD)酶聯免疫試劑盒
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貨號:CSB-E15051h
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規格:96T/48T
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價格:¥3600/¥2500
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其他:
產品詳情
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產品描述:人免疫球蛋白D(IgD)酶聯免疫試劑盒(CSB-E15051h)為雙抗夾心法ELISA試劑盒,定量檢測血清、血漿、組織勻漿樣本中的IgD含量。IgD為免疫球蛋白D,是B細胞抗原受體的重要組成部分。其背景上在人體免疫系統中發揮關鍵作用。研究機制方面,它參與B細胞的活化、增殖和分化過程,在免疫應答的啟動和調節中意義重大,目前對其功能機制仍在進一步深入研究。試劑盒檢測范圍為0.024 ng/mL-100 ng/mL,靈敏度為0.024 ng/mL。適用于免疫學基礎研究、炎癥機制探索或免疫相關疾病模型中IgD動態變化的定量分析;為免疫調控研究、藥物效應評估或生物標志物篩選提供可靠數據支持本品僅用于科研,不用于臨床診斷,產品具體參數及操作步驟詳見產品說明書。
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別名:N/A
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縮寫:IgD
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種屬:Homo sapiens (Human)
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樣本類型:serum, plasma, tissue homogenates
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檢測范圍:0.024 ng/mL-100 ng/mL
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靈敏度:0.024 ng/mL
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反應時間:1-5h
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樣本體積:50-100ul
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檢測波長:450 nm
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研究領域:Immunology
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測定原理:quantitative
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測定方法:Sandwich
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度:
To assess the linearity of the assay, samples were spiked with high concentrations of human IgD in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:100 Average % 91 Range % 87-98 1:200 Average % 95 Range % 90-102 1:400 Average % 93 Range % 87-99 1:800 Average % 94 Range % 86-105 -
回收率:
The recovery of human IgD spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 85-96 EDTA plasma (n=4) 92 88-96 -
標準曲線:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average Corrected 100 2.120 2.094 2.107 2.005 25 1.655 1.637 1.646 1.544 6.25 0.995 1.005 1.000 0.898 1.56 0.515 0.507 0.511 0.409 0.39 0.413 0.408 0.411 0.309 0.1 0.204 0.210 0.207 0.105 0.024 0.138 0.132 0.135 0.033 0 0.100 0.103 0.102 -
數據處理:
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貨期:3-5 working days
引用文獻
- Immunomodulatory constituents of human breast milk and immunity from bronchiolitis Chunyu Li.et al,Italian Journal of Pediatrics,2017
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靶點詳情
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最新研究進展:IgD是一種抗體分子,也是B淋巴細胞表面的一種受體。它在機體的免疫防御中起著重要作用,通過與抗原結合,誘導B細胞的激活和增殖,促進免疫應答的發生。最近的研究表明,IgD在機體的免疫調節中具有重要作用。一項研究發現,在人類B細胞中,IgD能夠與TLR7相互作用,從而抑制炎癥介質的產生。另一項研究發現,在小鼠模型中,IgD能夠抑制胰島素樣生長因子1(IGF1)的信號通路,從而降低肥胖相關的代謝疾病的發生率。
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