Human Hyperglycosylated Chorionic Gonadotropin(hCG) ELISA Kit

Human Hyperglycosylated Chorionic Gonadotropin(hCG) ELISA Kit

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中文名稱：

人高糖基化人絨毛膜促性腺激素(hCG)酶聯(lián)免疫試劑盒
貨號：

CSB-E15803h
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人高糖基化人絨毛膜促性腺激素(hCG)酶聯(lián)免疫試劑盒（CSB-E15803h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、尿液、組織培養(yǎng)上清液樣本中的hCG含量。hCG即人絨毛膜促性腺激素，是妊娠時胎盤滋養(yǎng)層細(xì)胞分泌的糖蛋白。在醫(yī)學(xué)研究中，其可用于妊娠診斷。研究機(jī)制上，它能促進(jìn)黃體發(fā)育，維持妊娠狀態(tài)，還和腫瘤發(fā)生發(fā)展相關(guān)，在腫瘤診斷和治療靶點研究受關(guān)注。試劑盒檢測范圍為0.67 mIU/mL-20 mIU/mL，該產(chǎn)品適用于生殖生物學(xué)研究，如妊娠相關(guān)分子機(jī)制探索、滋養(yǎng)層細(xì)胞功能分析，也可用于腫瘤生物學(xué)領(lǐng)域中對hCG異常表達(dá)的機(jī)制研究，或體外細(xì)胞模型中激素分泌水平的動態(tài)監(jiān)測。通過標(biāo)準(zhǔn)化的實驗流程，為科研工作者提供穩(wěn)定可靠的數(shù)據(jù)支持，滿足生殖醫(yī)學(xué)、細(xì)胞生物學(xué)及疾病模型構(gòu)建等領(lǐng)域的高靈敏度檢測需求。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

N/A
縮寫：

hCG
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, urine, cell culture supernates
檢測范圍：

0.67 mIU/mL-20 mIU/mL
靈敏度：

0.167 mIU/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human hCG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	97
	Range %	92-103
1:4	Average %	96
	Range %	90-100
1:8	Average %	95
	Range %	89-101

回收率：

The recovery of human hCG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	91-103
EDTA plasma (n=4)	94	89-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
20	2.542	2.539	2.541	2.493
8.3	1.282	1.276	1.279	1.231
3.3	0.642	0.634	0.638	0.590
1.3	0.288	0.285	0.287	0.239
0.67	0.156	0.149	0.153	0.105
0	0.049	0.047	0.048

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Evaluating the ability of an oocyte to repair fragmented sperm chromatin Keating D, et al,Fertility and Sterility,2019
Maternal age and blastocyst quality do not influence the embryo production of hyperglycosylated human chorionic gonadotropin Parvanov D, et al,Fertility and Sterility,2019
Comparative analysis of DNA methylation in euploid and aneuploid human embryos using reduced representation bisulfite sequencing Tao X, et al,Fertility and Sterility,2019

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.