Human Hydroxyacid oxidase 1(HAO1) ELISA kit

說明書下載

中文名稱：

人羥基酸氧化酶1(HAO1)酶聯免疫試劑盒
貨號：

CSB-EL010127HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人羥基酸氧化酶1(HAO1)酶聯免疫試劑盒（CSB-EL010127HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的HAO1含量。HAO1，即羥基酸氧化酶1，是一種FMN依賴的α-羥基酸脫氫酶家族成員，主要在肝臟的過氧化物酶體中氧化甘醇酸生成甘醇酸內酯，同時產生過氧化氫。其表達在氧化應激期間可能下調，以防止過氧化氫在過氧化物酶體中過量生成，可能是一種對氧化損傷的響應機制。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，適用于科研場景中HAO1的功能研究，如代謝性疾病模型構建、肝腎組織病理分析、藥物作用靶點篩選等方向，為探索代謝調控機制及疾病干預策略提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

(S) 2 hydroxy acid oxidase ELISA Kit; Glycolate oxidase ELISA Kit; GOX ELISA Kit; GOX1 ELISA Kit; HAO1 ELISA Kit; HAOX1 ELISA Kit; HAOX1_HUMAN ELISA Kit; Hydroxyacid oxidase 1 ELISA Kit; MGC142225 ELISA Kit; MGC142227 ELISA Kit; OTTHUMP00000030231 ELISA Kit
縮寫：

HAO1
Uniprot No.：

Q9UJM8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.69 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human HAO1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	98
	Range %	93-112
1:10	Average %	107
	Range %	103-109
1:20	Average %	93
	Range %	90-97
1:40	Average %	88
	Range %	86-94

回收率：

The recovery of human HAO1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	99-106
EDTA plasma (n=4)	92	90-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
1200	2.193	2.369	2.281	2.097
600	1.700	1.710	1.705	1.521
300	1.253	1.262	1.258	1.074
150	0.878	0.898	0.888	0.704
75	0.532	0.566	0.549	0.365
37.5	0.411	0.422	0.417	0.233
18.75	0.231	0.222	0.227	0.043
0	0.177	0.190	0.184

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Has 2-hydroxyacid oxidase activity. Most active on the 2-carbon substrate glycolate, but is also active on 2-hydroxy fatty acids, with high activity towards 2-hydroxy palmitate and 2-hydroxy octanoate.
基因功能參考文獻：
1. The study presents the first spectroscopic evidence of the formation of an intermediate with absorbance features resembling those of a flavosemiquinone in the oxidative half-reaction of glycolate oxidase. PMID: 21141873
2. The inhibitor heteroatoms interact with five active-site residues that have been implicated in catalysis in homologous flavodehydrogenases of L-2-hydroxy acids. In addition, the chlorophenyl substituent is surrounded by nonconserved hydrophobic residues. PMID: 20054120
3. These inhibitions suggest that glycolate binds to the active site of the reduced enzyme, and that DCIP also has affinity for the oxidized enzyme. PMID: 17669354
4. Active site and loop 4 movements within human glycolate oxidase. PMID: 18215067
亞細胞定位：

Peroxisome.
蛋白家族：

FMN-dependent alpha-hydroxy acid dehydrogenase family
組織特異性：

Liver.
數據庫鏈接：

HGNC: 4809

OMIM: 605023

KEGG: hsa:54363

STRING: 9606.ENSP00000368066

UniGene: Hs.193640

Human Hydroxyacid oxidase 1(HAO1) ELISA kit

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