Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

文獻引用 (5) 評價 (1) 說明書下載

中文名稱：

人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶聯免疫試劑盒
貨號：

CSB-EL007812HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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人HERV-K_7p22.1前病毒祖先Env多蛋白(ERVK6)酶聯免疫試劑盒（CSB-EL007812HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的ERVK6含量。人HERV-K_7p22.1前病毒祖先Env多蛋白（ERVK6）是內源性逆轉錄病毒K組的一個成員，其Env蛋白在早期感染中參與受體識別和膜融合。ERVK6蛋白在血清、血漿和組織勻漿中定量測量，具有潛在的研究價值。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于體外樣本中ERVK6蛋白表達水平的精準分析，可為病毒進化研究、宿主基因組穩定性探索以及相關疾病模型的分子機制解析提供技術支持。該產品嚴格適用于科研場景，包括但不限于細胞實驗、動物模型或生物樣本庫中ERVK6蛋白的動態監測，為分子生物學及病毒學領域的研究者提供穩定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ERVK-6 ELISA Kit; ERVK6Endogenous retrovirus group K member 6 Env polyprotein ELISA Kit; EnvK2 protein ELISA Kit; Envelope polyprotein ELISA Kit; HERV-K(C7) envelope protein ELISA Kit; HERV-K(HML-2.HOM) envelope protein ELISA Kit; HERV-K108 envelope protein ELISA Kit; HERV-K_7p22.1 provirus ancestral Env polyprotein) [Cleaved into: Surface protein ELISA Kit; SU); Transmembrane protein ELISA Kit; TM)] ELISA Kit
縮寫：

ERVK6
Uniprot No.：

Q69384
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human ERVK6 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-99
1:2	Average %	103
	Range %	98-106
1:4	Average %	110
	Range %	106-114
1:8	Average %	92
	Range %	89-97

回收率：

The recovery of human ERVK6 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	81-89
EDTA plasma (n=4)	97	93-100

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.251	2.301	2.276	2.154
5	1.375	1.368	1.372	1.250
2.5	0.782	0.778	0.780	0.658
1.25	0.503	0.511	0.507	0.385
0.625	0.325	0.336	0.331	0.209
0.312	0.247	0.268	0.258	0.136
0.156	0.192	0.186	0.189	0.067
0	0.121	0.123	0.122

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Corrigendum: Subtype-specific human endogenous retrovirus K102 envelope protein is a novel serum immunosuppressive biomarker of cancer Q Gong, R Xu,Frontiers in Immunology,2025
Subtype-specific HERV-K102 envelope is a novel serum immunosuppressive biomarker and therapeutic target of cancer R Xu,/,2024
Human endogenous retroviruses are upregulated in Frontotemporal Dementia-MAPT mutation carriers S Han,/,2024
Nuclear lamina erosion-induced resurrection of endogenous retroviruses underlies neuronal aging H Zhang,Cell reports,2023
Human endogenous retrovirus K (HERV-K) env in neuronal extracellular vesicles: a new biomarker of motor neuron disease Y Li,Amyotrophic Lateral Sclerosis and Frontotemporal Degeneration,2021

產品評價

平均分:

5.0分 - 1 個評價

樣品類型：血清

樣品信息：人

稀釋比：沒有稀釋

產品評價：挺好的，靈敏度很高，精確度也高，實驗結果很好，是陽性數據，擬合曲線也挺好，試劑盒很好用，操作挺簡單的

By 肖老師

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功能：

Retroviral envelope proteins mediate receptor recognition and membrane fusion during early infection. Endogenous envelope proteins may have kept, lost or modified their original function during evolution. This endogenous envelope protein has lost its original fusogenic properties.; SU mediates receptor recognition.; TM anchors the envelope heterodimer to the viral membrane through one transmembrane domain. The other hydrophobic domain, called fusion peptide, mediates fusion of the viral membrane with the target cell membrane.
亞細胞定位：

[Transmembrane protein]: Cell membrane; Single-pass type I membrane protein.; [Surface protein]: Cell membrane; Peripheral membrane protein.; [Endogenous retrovirus group K member 6 Env polyprotein]: Virion.
蛋白家族：

Beta type-B retroviral envelope protein family, HERV class-II K(HML-2) env subfamily
組織特異性：

Expressed in testis, and peripheral blood lymphocytes.
數據庫鏈接：

HGNC: 13915

OMIM: 605626

Human HERV-K_7p22.1 provirus ancestral Env polyprotein(ERVK6) ELISA kit

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