Human Granulocyte-Macrophage Colony Stimulating Factor,GM-CSF ELISA Kit
產(chǎn)品詳情
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產(chǎn)品描述：
人粒細(xì)胞巨噬細(xì)胞集落刺激因子(GM-CSF)酶聯(lián)免疫試劑盒（CSB-E04568h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織培養(yǎng)上清液、組織勻漿樣本中的CSF2含量。CSF2即集落刺激因子2，在免疫調(diào)節(jié)等生理過程中發(fā)揮重要作用。其背景在于能促進(jìn)造血干細(xì)胞向粒細(xì)胞和巨噬細(xì)胞等分化。研究機(jī)制方面，主要是通過與細(xì)胞表面相應(yīng)受體結(jié)合，激活細(xì)胞內(nèi)信號(hào)通路，進(jìn)而調(diào)控細(xì)胞增殖、分化等生物學(xué)行為。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于體外實(shí)驗(yàn)研究，如探究炎癥反應(yīng)機(jī)制、評(píng)估藥物對(duì)免疫細(xì)胞因子分泌的影響，或分析疾病模型中GM-CSF的動(dòng)態(tài)變化本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
Colony stimulating factor 2 (granulocyte-macrophage) ELISA Kit; Colony Stimulating Factor 2 ELISA Kit; Colony stimulating factor ELISA Kit; Colony-stimulating factor ELISA Kit; CSF 2 ELISA Kit; CSF ELISA Kit; CSF2 ELISA Kit; CSF2_HUMAN ELISA Kit; GM-CSF ELISA Kit; GMCSF ELISA Kit; Granulocyte Macrophage Colony Stimulating Factor ELISA Kit; Granulocyte-macrophage colony-stimulating factor ELISA Kit; MGC131935 ELISA Kit; MGC138897 ELISA Kit; MGI1GM ELISA Kit; Molgramostin ELISA Kit; Pluripoietin-a ELISA Kit; Sargramostim ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, cell culture supernates, tissue homogenates
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檢測(cè)范圍：
15.6 pg/mL-1000 pg/mL
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靈敏度：
3.9 pg/mL
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反應(yīng)時(shí)間：
1-5h
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樣本體積：
50-100ul
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檢測(cè)波長：
450 nm
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研究領(lǐng)域：
Immunology
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測(cè)定原理：
quantitative
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測(cè)定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% |
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| Three samples of known concentration were tested twenty times on one plate to assess. |
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| Inter-assay Precision (Precision between assays): CV%<10% |
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| Three samples of known concentration were tested in twenty assays to assess. |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human GM-CSF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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Sample |
Serum(n=4) |
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| 1:1 |
Average % |
89 |
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| Range % |
82-99 |
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| 1:2 |
Average % |
95 |
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| Range % |
88-102 |
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| 1:4 |
Average % |
95 |
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| Range % |
86-99 |
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| 1:8 |
Average % |
95 |
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| Range % |
90-105 |
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回收率：
| The recovery of human GM-CSF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type |
Average % Recovery |
Range |
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| Serum (n=5) |
92 |
86-100 |
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| EDTA plasma (n=4) |
96 |
90-110 |
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標(biāo)準(zhǔn)曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| pg/ml |
OD1 |
OD2 |
Average |
Corrected |
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| 1000 |
2.987 |
2.913 |
2.950 |
2.792 |
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| 500 |
2.417 |
2.429 |
2.423 |
2.265 |
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| 250 |
1.674 |
1.686 |
1.680 |
1.522 |
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| 125 |
0.954 |
0.963 |
0.959 |
0.801 |
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| 62.5 |
0.576 |
0.562 |
0.569 |
0.411 |
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| 31.2 |
0.386 |
0.394 |
0.390 |
0.232 |
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| 15.6 |
0.261 |
0.268 |
0.265 |
0.107 |
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| 0 |
0.156 |
0.159 |
0.158 |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human GM-CSF. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled GM-CSF antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻(xiàn)
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Upregulation of Circ_0035266 Contributes to the Malignant Progression of Inflammation-Associated Malignant Transformed Cells Induced by Tobacco-Specific Carcinogen NNK
Q Hua,Toxicological sciences,2023
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The TLR4/NF-κB signaling pathway-mediated type 2 skewing of T helper cell in cough variant asthma was counteracted by ethanol extract of Anacyclus pyrethrum root
J Zheng,Immunobiology,2023
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Increased percentage of PD-L1+ natural killer cells predicts poor prognosis in sepsis patients: a prospective observational cohort study
W Jiang,Critical Care,2020
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Plasma Neurofilament Light Chain May Be a Biomarker for the Inverse Association Between Cancers and Neurodegenerative Diseases
S Liu,Front Aging Neurosci,2020
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Kruppel like factor 6 splice variant 1 (KLF6-SV1) overexpression recruits macrophages to participate in lung cancer metastasis by up-regulating TWIST1
Wang J, et al,Cancer Biology &Therapy,2018
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PKCa in colon cancer cells promotes M1 macrophage polarization via MKK3/6-\P38 MAPK pathway
Yang Cheng .et al,MOL CARCINOGEN,2018
靶點(diǎn)詳情
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最新研究進(jìn)展：
CSF2（集落刺激因子2），也稱為粒細(xì)胞巨噬細(xì)胞集落刺激因子（GM-CSF），是一種細(xì)胞因子，主要由T細(xì)胞、B細(xì)胞、巨噬細(xì)胞和肺上皮細(xì)胞等多種細(xì)胞類型產(chǎn)生。它在免疫系統(tǒng)中扮演著促進(jìn)巨噬細(xì)胞、樹突狀細(xì)胞和粒細(xì)胞分化、增殖和功能發(fā)揮的重要作用。目前，研究者們正在探究CSF2在許多疾病中的潛在作用，例如自身免疫疾病、腫瘤和感染等方面的作用，并正在研發(fā)針對(duì)CSF2的治療藥物。
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功能：
Cytokine that stimulates the growth and differentiation of hematopoietic precursor cells from various lineages, including granulocytes, macrophages, eosinophils and erythrocytes.
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基因功能參考文獻(xiàn)：
- GM-CSF could be induced by M. pneumoniae infection in vivo and vitro. Childen with high level GM-CSF had longer duration of fever. GM-CSF probably plays a vital role in neutrophil inflammation in M. pneumoniae infection. PMID: 30007865
- Data show that CD4 T cells from synovial fluid are enriched for granulocyte-macrophage colony-stimulating factor (GM-CSF) production, suggests that the GM-CSF programme may be a primary pathogenic process. PMID: 29142230
- GM-CSF bioavailability plays a critical role in maintaining intestinal homeostasis. Decreased bioavailability coupled with the genetic risk markers and/or smoking results in aggressive ileal Crohn's disease behavior. PMID: 29434451
- Data show that GIF (ORF117 ) serves as a competitive decoy receptor by leveraging binding hotspots underlying the cognate receptor interactions of granulocyte macrophage colony-stimulating factor (GM-CSF) and interleukin-2 (IL-2). PMID: 27819269
- As translated to human disease, these findings suggest vaccine-mediated expansion of GM-CSF-producing T cells could be an effective prophylactic or therapeutic TB strategy. PMID: 29233902
- In a subset of patients who received GM-CSF, circulating myeloid-derived suppressor cells (MDSC), and anti-GM-CSF-neutralizing antibodies (Nabs) were also modulated. The majority of patients developed anti-GM-CSF Nabs, which correlated with improved RFS and OS PMID: 28536308
- this study shows that cortisol inhibits CSF2 via DNA methylation and inhibits invasion in first-trimester trophoblast cells PMID: 28846166
- Oral and periodontal innate immunity is affected by HIV viremia and ART. GCF IL-8, G-CSF, as well as serum IL-8, MCP-1 and GM-CSF may be useful biomarkers for the detection of disease presence and/or its severity due to HIV infection and ART use. PMID: 28043030
- This review summarizes a number of findings to provide the currently available information regarding the anticancer immune response of GM-CSG. [review] PMID: 27364892
- Data suggest that the intratumoral GM-CSF expression, as a potentially independent prognostic biomarker for recurrence, might improve conventional clinical and pathologic analysis to refine outcome prediction for clinically localized clear-cell renal cell carcinoma (ccRCC) patients after surgery. PMID: 27016418
- High GM-CSF expression is associated with breast Cancer. PMID: 28754674
- In gastric cancer (GC), tumour-derived GM-CSF activated neutrophils and induced neutrophil PD-L1 expression via Janus kinase (JAK)-signal transducer and activator of transcription 3 (STAT3) signalling pathway. The activated PD-L1(+) neutrophils effectively suppressed normal T-cell immunity in vitro and contributed to the growth and progression of human GC in vivo. PMID: 28274999
- The IL-3/ GM-CSF effected on the myofibroblastic differentiation of human adipose derive stromal cells (hASCs) as well as it did on human dermal fibroblasts (HDFs). PMID: 28377320
- Obesity alters the lung neutrophil infiltration to enhance breast cancer metastasis through IL5 and GM-CSF. PMID: 28737771
- Data suggest that the methylotrophic yeast Pichia pastoris is an effective recombinant host for heterologous granulocyte-macrophage colony-stimulating factor (rhGM-CSF) production. PMID: 28376863
- Letter: Knockdown of either filaggrin or loricrin increases the productions of interleukin (IL)-1alpha, IL-8, IL-18 and granulocyte macrophage colony-stimulating factor in stratified human keratinocytes. PMID: 26381575
- Impaired RASGRF1/ERK-mediated GM-CSF response characterizes CARD9 deficiency in French-Canadians. PMID: 26521038
- Honokiol could possess potential anti-inflammatory effects and inhibits TNF-alpha-induced IL-1beta, GM-CSF and IL-8 production in PBMCs from rheumatoid arthritis patients. PMID: 26339358
- The canonical NFkappaB signaling in fibroblasts, but not in tumor cells, was shown to be responsible for the induced and constitutive CSF2 expression. PMID: 25919140
- GM-CSF primes IL-13 production by macrophages via PAR-2. PMID: 25633855
- analysis of an IL2RA polymorphism that controls GM-CSF production in human TH cells PMID: 25278028
- NFKBIZ gene knockdown in bronchial epithelial cells suppresses the release of IL-1b-induced IL-6 and GMCSF. PMID: 25629767
- CFH Y402H polymorphism is associated with elevated vitreal GM-CSF and choroidal macrophages in the postmortem human eye. PMID: 25814824
- Influenza infection stimulates the secretion of IL-8 and GM-CSF by alveolar epithelial cells. PMID: 26033355
- Periodic fever, aphthous stomatitis, pharyngitis and cervical adenopathy syndrome febrile episodes are characterized by activation of GM-CSF and IL-8 with Th1 skewing. PMID: 24670131
- activation of STAT3 induces the expression of GM-CSFRa that protects CLL cells from apoptosis, suggesting that inhibition of STAT3 or GM-CSFRa may benefit patients with chronic lymphocytic leukemia PMID: 24836891
- G-CSF- or GM-CSF-secreting cancers, albeit not very common are, however, among the most rapidly advancing ones due to a cytokine-mediated immune suppression and angiogenesis. [Review] PMID: 24692240
- Treatment with GM-CSF increased the expression of vimentin and ZEB1, similar to TGF-b1 treatment, and decreased the expression of E-cadherin. Our results suggest that GM-CSF could induce EMT in human HERS/ERM cells. PMID: 24258001
- GM-CSF polymorphism may be involved in the pathogenesis of atopic dermatitis. PMID: 24117406
- The association of GM-CSF expression with systemic inflammation highlights the potential role of Th17-related cytokines in the pathology of juvenile idiopathic arthritis. PMID: 24692225
- M-CSF induces macrophage transformation by upregulating c-Jun with a certain synergy of NFkappaB. PMID: 24100343
- The detection of basal level of IgM rheumatoid factor, IgM and also certain cytokines can be useful in prognosis of effectiveness of rituximab therapy under rheumatoid arthritis. PMID: 25080789
- Cytokines and signaling molecules predict clinical outcomes in sepsis. PMID: 24244449
- Granulocyte colony-stimulating factor produces long-term changes in gene and microRNA expression profiles in CD34+ cells from healthy donors. PMID: 24056818
- mesenchymal-like breast cancer cells activate macrophages to a tumor-associated macrophage-like phenotype by GM-CSF. PMID: 24823638
- The comparison between the cytokine profiles of ET and PV patients showed a statistically significant increase of IL-4, IL-8, GM-CSF, interferon -gamma, MCP-1, PDGF-BB, and VEGF in the ET group PMID: 24463275
- Data indicate that differentially expressed IL-17, IL-22, and IL-23 levels are associated with K-ras in a stage-specific fashion along colorectal cancer progression and an association was established between mutant K-ras and GM-CSF and IFN-gamma. PMID: 24040001
- in the type 2 diabetes mellitus group, significantly higher IL-4 level on the first and the third post dengue virus infection days, while higher IL-10 and granulocyte-macrophage colony-stimulating factor were detected on the third postinfection day. PMID: 24078930
- loss of BMPR2 induced prolonged phospho-p38 MAPK in response to TNF, and this increased GADD34-PP1 phosphatase activity, dephosphorylating eukaryotic translation initiation factor (eIF2alpha), and derepressing GM-CSF mRNA translation PMID: 24446489
- Since GM-CSF plays an important role during pregnancy, the reduced levels of GM-CSF in the cervico-vaginal fluid of human papillomavirus(+) women might contribute to explain the reduced live birth rate. PMID: 23920355
- SECTM1 secreted from bone marrow stromal cells may interact with CD7 to influence GM-CSF expression in leukemic cells. PMID: 24211252
- Describe GM-CSF ELISA for routine clinical diagnosis of autoimmune pulmonary alveolar proteinosis. PMID: 24275678
- The data presented suggest that -677C/A in the promoter region of the GM-CSF gene does not play a role in acne pathogenesis. PMID: 23069312
- CBL linker and RING finger mutants lead to enhanced GM-CSF signaling due to elevated kinase expression, which can be blocked using small molecule inhibitors targeting specific downstream pathways. PMID: 23696637
- impairment of NFATC2-calcineurin interaction promotes megakaryocyte proliferation through up-regulation of CSF2 transcription PMID: 22911897
- Overexpression of a nonphosphorylatable MT1-MMP mutant (Y573F) abrogates CSF-2 and CSF-3 transcriptional increases. PMID: 23548906
- GM-CSF contributes to aortic aneurysms resulting from SMAD3 deficiency PMID: 23585475
- Within pediatric Crohn disease, neutrophil GM-CSF-dependent STAT5 activation and bacterial killing were reduced as GM-CSF autoantibodies increased. PMID: 23600834
- This study demonistrated that GMCSF is downregulation in brain in patient with Alzheimer's disease. PMID: 22430742
- IL-27 suppresss GM-CSF production under nonpolarizing and T helper (Th)1 cell, but not T helper (Th)17 cell conditions, in both murine and human cultures. PMID: 22837488
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亞細(xì)胞定位：
Secreted.
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蛋白家族：
GM-CSF family
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數(shù)據(jù)庫鏈接：
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