產(chǎn)品詳情
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產(chǎn)品描述：
人凝溶膠蛋白(Gelsolin)酶聯(lián)免疫試劑盒（CSB-E08160h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GSN含量。人凝溶膠蛋白（Gelsolin）是一種廣泛存在于細胞、血液和腦脊液中的多功能肌動蛋白結(jié)合蛋白，其經(jīng)典功能是調(diào)節(jié)肌動蛋白絲的聚合、解聚和剪切，在細胞骨架結(jié)構(gòu)重排和細胞運動中發(fā)揮重要作用。最新研究發(fā)現(xiàn)，Gelsolin與腫瘤和阿爾茨海默癥等疾病相關(guān)，其表達量在多種癌變組織中呈下降趨勢，且可能通過抑制癌細胞生長來抑制腫瘤生長和轉(zhuǎn)移。試劑盒檢測范圍為2.74 ng/ml- 2000 ng/ml，適用于探索Gelsolin在細胞生物學(xué)中的功能機制、疾病模型中的表達變化以及作為潛在生物標(biāo)志物的分析，可為神經(jīng)科學(xué)、腫瘤學(xué)及免疫學(xué)等基礎(chǔ)研究提供可靠工具本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
Actin depolymerizing factor ELISA Kit; Actin-depolymerizing factor ELISA Kit; ADF ELISA Kit; AGEL ELISA Kit; Brevin ELISA Kit; DKFZp313L0718 ELISA Kit; GELS_HUMAN ELISA Kit; Gelsolin ELISA Kit; Gsn ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, plasma, tissue homogenates
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檢測范圍：
2.74 ng/ml - 2000 ng/ml
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靈敏度：
2.74 ng/ml
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Metabolism
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human gelsolin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
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| Sample | Serum(n=4) | |
1:1 | Average % | 95 | |
Range % | 90-103 | |
1:2 | Average % | 94 | |
Range % | 88-100 | |
1:4 | Average % | 90 | |
Range % | 86-95 | |
1:8 | Average % | 91 | |
Range % | 85-99 | |
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回收率：
The recovery of human gelsolin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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Sample Type | Average % Recovery | Range | |
Serum (n=5) | 95 | 92-98 | |
EDTA plasma (n=4) | 98 | 93-106 | |
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標(biāo)準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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| | | | | | |
ng/ml | OD1 | OD2 | Average | Corrected | |
2000 | 2.735 | 2.765 | 2.750 | 2.665 | |
666.66 | 1.995 | 2.130 | 2.063 | 1.978 | |
222.22 | 1.358 | 1.334 | 1.346 | 1.261 | |
74.07 | 0.747 | 0.751 | 0.749 | 0.664 | |
24.69 | 0.461 | 0.435 | 0.448 | 0.363 | |
8.23 | 0.232 | 0.223 | 0.228 | 0.143 | |
2.74 | 0.191 | 0.195 | 0.193 | 0.108 | |
0 | 0.086 | 0.084 | 0.085 | | |
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本試劑盒所含材料：
- A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human GSN antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labeled GSN antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibodyDiluent (15 ml/bottle) ---Dilute the Biotin-antibody.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the HRP-avidin solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
靶點詳情
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功能：
Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
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基因功能參考文獻：
- the gelsolin N184K variant is subjected to the same aberrant proteolytic events responsible for the formation of amyloidogenic fragments in the previously characterized mutants. PMID: 27633054
- Induction of cell stress alters the actin cytoskeleton in intestinal epithelial cells via changes in the actin-binding proteins villin-1 and gelsolin. PMID: 29274870
- Gelsolin upregulation promotes radioresistance in non-small cell lung cancer cells, at least partially, through activation of phosphoinositide 3-kinase/Akt signaling. PMID: 27121073
- Results identify GSN as target of miR-200a which inhibits the release of microvesicles in hepatocellular carcinoma cells by functionally regulating GSN. PMID: 28440466
- Gelsolin enhances the invasive capacity of colon cancer cells via elevating intracellular superoxide (O2.-) levels by interacting with Cu/ZnSOD, and gelsolin gene expression positively correlates with urokinase plasminogen activator (uPA), an important matrix-degrading protease invovled in cancer invasion. PMID: 27391159
- these data profile the importance of the physical interaction between HPV16 E7 and gelsolin in the acquisition of the metastatic phenotype by cervical cancer cells PMID: 27072581
- Our study highlights gelsolin as an important pro-disseminative factor contributing to the aggressive phenotype of diffuse gastric cancer PMID: 27058427
- study strongly supported the contribution of the genes ITGA2B, GSN and RHOA and the two pathways "regulation of actin cytoskeleton" and "leukocyte transendothelial migration" to osteoporosis risk. PMID: 27153759
- SSH1 binds to gelsolin via actin filaments in the cytosolic fraction. Gelsolin promoted solubilization of actin filaments and SSH1 in cell-free assays and in cultured cells. PMID: 25451266
- free actin likely contributes to impaired host defense by blocking scavenger receptor binding of bacteria. PMID: 28385809
- analysis of how YopO disrupts normal gelsolin function to alter host actin dynamics and thus cripple phagocytosis PMID: 28280241
- Our present study implied that the synergistic effect of pGSN and pIgA induced glomerular fibrosis via the TGF-beta1/Smads signal transduction pathway. This might be a potential mechanism for the glomerular fibrosis observed in IgAN patients. PMID: 28208683
- The expression levels of GSN were observed to be reduced in colon carcinoma (CC) cells, and the reduced expression level of GSN was often associated with a poorer metastasisfree survival rate in patients with CC (P=0.04). In addition, the overexpression of GSN inhibited the invasion of CC cells in vitro. PMID: 27573444
- in chronic hemodialysis patients, lower pGSN levels were not associated with hospitalization, all-cause and cardio-vascular mortality, even though pGSN was inversely correlated with age, CRP and IL-6, suggesting that inflammation may influence pGSN PMID: 28114138
- PODXL enhances motility and invasiveness through an increase in gelsolin-actin interactions in cell protrusions. PMID: 27461278
- These findings suggest that gelsolin may have a role in the disease process in atopic dermatitis patients. PMID: 26318415
- Plasma GSN may play an important role in the development of immunoglobulin A (IgA) nephropathy. PMID: 27997897
- beta-Catenin seems to be involved in the regulation of gelsolin expression, which in turn affects the migratory ability of colonic cancer cells PMID: 27798885
- hypoxia or GSN overexpression induces HIF-1alpha expression and reduces the expression of survival markers p-Akt and Bcl-2 in H9c2 cardiomyoblast cells PMID: 27193608
- Increasing amount of amyloid are associated with the severity of clinical features in hereditary gelsolin amyloidosis. PMID: 27879149
- The rs1078305 and rs10818524 SNPs of GSN were associated with increased risk for oral squamous cell carcinoma development in a Chinese Han population PMID: 26848502
- Lower blood levels of gelsolin associated with progressive aortic arch calcification. PMID: 26941566
- Gelsolin protein, human, yielded a receiver operating characteristics value of 89% for node-positive OSCC PMID: 25223295
- GSN overexpression suppresses apoptosis while down-regulated GSN promotes apoptosis in hepatocellular carcinoma. PMID: 26823700
- Fragmented gelsolins may be associated with the pathogenesis of fibrosis in RA-ILD. PMID: 26666486
- gelsolin interacts with particular components of the three cytoskeleton systems and is a constituent of midbodies PMID: 26598132
- novel roles for actin-depolymerizing factor and cofilin-1 in regulating the remodeling and permeability of epithelial junctions PMID: 26878213
- The overexpression of GSN may inhibit the proliferation, adhesion ability and invasion of 786-0 clear cell renal cell carcinoma cell line. PMID: 26398833
- Serum-expressed apolipoprotein B-100 protein, C9 Complement, and gelsolin can be used for differential diagnosis of Barrertts esophagus and adenocarcinoma of esophagus. PMID: 26404905
- TGF-beta1 induced epigenetic modification of GSN could alter the EMT process in breast cancer cells. PMID: 26482896
- GSNs might play a regulatory role in the suppression of the tissue damage induced by acute radiation exposure PMID: 25164111
- We designed and synthesized siRNA against various exons in the gelsolin gene (GSN)..our finds reveal siRNA, which is derived from target gene exon, can form the complex with H3 histone to be involved in the regulation of gene expression PMID: 25600697
- Results show that high gelsolin levels are associated with better prognosis in ER+HER2- breast cancer and a reduction in tumor cell migration. PMID: 26408687
- Gelsolin expression promoted tumor-associated phenotypes by facilitating proliferative and invasive capacities of hepatocellular carcinoma(HCC) cells, which might serve as a potential therapeutic target for HCC treatment. PMID: 26149653
- Based on the obtained results, we propose that the gelsolin is an important cellular target for cotinine, through which this compound influences on the basic processes involved in neoplastic transformation and metastasis, such as migration and apoptosis PMID: 25544037
- Group B Streptococcus-beta-haemolysin is solely responsible for gelsolin increase causing, through membrane permeability defects, calcium influx and calpain activation. PMID: 25130983
- Plasma gelsolin levels decrease in the blood of type II diabetics. Recombinant gelsolin helped in improving glycemic control in diabetic mice. PMID: 25478578
- Data show that gelsolin (GSN) plays an important role in the regulation of gynecological cell fate as reflected in dysregulation in chemosensitivity. PMID: 25246592
- Data indicate gelsolin in contributing to the invasive potential of LNCaP prostate adenocarcinoma cells. PMID: 25581609
- Gelsolin levels were a useful tool to predict functional outcome and mortality after aneurysmal subarachnoid hemorrhage. PMID: 23880145
- increased plasma levels after allergen-specific immunotherapy PMID: 24980225
- Patients developing cardiopulmonary bypass acute lung injury had lower plasma gelsolin reservoir and a much more amount and rapid consumption of plasma gelsolin early after operation PMID: 25126004
- This study improves the knowledge of the genetic features of Mexican patients with corneal stromal dystrophies by identifying mutations in the TGFBI, CHST6, and GSN genes. PMID: 24801599
- GSN is important for chemoresistance in head-and-neck cancer PMID: 24771612
- we predict that the gene silencing of GSN and/or the downstream blocking of GSN along the p38 pathway could be applied to ameliorate pathological cardiac hypertrophy in the future PMID: 24505034
- Ceruloplasmin and gelsolin are closely interacted with the oncogene NF-kappab. PMID: 23925487
- Plasma gelsolin is lower in normal pregnancy than in non-pregnant women. It is also significantly lower in pre-eclampsia than in normal pregnancy. PMID: 24239294
- This study found significantly lower plasma gelsolin levels in patients with systemic lupus erythematosus and rheumatoid arthritis compared with healthy controls PMID: 24122723
- Expression of mutant form of human gelsolin in mice under the control of a muscle-specific promoter induced myopathic changes reminiscent of human inclusion body myositis. PMID: 24047347
- Gelsolin can constitute a barrier that restricts HIV-1 infection of CD4+ lymphocytes in a pre-fusion step. PMID: 23575248
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相關(guān)疾病：
Amyloidosis 5 (AMYL5)
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亞細胞定位：
[Isoform 2]: Cytoplasm, cytoskeleton.; [Isoform 1]: Secreted.
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蛋白家族：
Villin/gelsolin family
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組織特異性：
Phagocytic cells, platelets, fibroblasts, nonmuscle cells, smooth and skeletal muscle cells.
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數(shù)據(jù)庫鏈接：
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