Human Galanin-like peptide(GALP) ELISA kit

Human Galanin-like peptide(GALP) ELISA kit

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文獻引用 (2) 說明書下載

中文名稱：

人甘丙肽樣肽(GALP)酶聯免疫試劑盒
貨號：

CSB-EL009222HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人甘丙肽樣肽(GALP)酶聯免疫試劑盒（CSB-EL009222HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GALP含量。GALP（甘丙肽樣肽）是一種神經肽。其背景上，它在中樞神經系統等有表達。研究機制方面，GALP與甘丙肽受體相互作用，參與調節能量代謝、生殖功能、攝食行為等生理過程，在生物體內發揮著重要的神經調節作用。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，為體外實驗研究其生理病理機制提供可靠工具，尤其適用于探究代謝調控、神經內分泌網絡及相關疾病模型的分子機制，滿足基礎科研中對GALP表達水平動態監測的需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

GALP ELISA Kit; Galanin-like peptide ELISA Kit
縮寫：

GALP
Uniprot No.：

Q9UBC7
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human GALP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	87-97
1:2	Average %	101
	Range %	97-103
1:4	Average %	95
	Range %	88-98
1:8	Average %	99
	Range %	94-102

回收率：

The recovery of human GALP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-102
EDTA plasma (n=4)	94	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.057	2.080	2.069	1.918
5	1.761	1.812	1.787	1.636
2.5	1.403	1.373	1.388	1.237
1.25	0.988	0.973	0.981	0.830
0.625	0.703	0.727	0.715	0.564
0.312	0.477	0.497	0.487	0.336
0.156	0.322	0.331	0.327	0.176
0	0.150	0.152	0.151

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Alarin: A Novel Circulating Peptide Hormone Linked to Luteinizing Hormone and Hiperandrogenismin Polycystic Ovary Syndrome Merve Bicer, et al,Current opinion in obstetrics & gynecology,2018
Kisspeptin And Galanin-like Peptide (GALP) Levels In Women With Polycystic Ovary Syndrome PV Nyagolova.et al,/,/

功能：

Hypothalamic neuropeptide which binds to the G-protein-coupled galanin receptors (GALR1, GALR2 and GALR3). Involved in a large number of putative physiological functions in CNS homeostatic processes, including the regulation of gonadotropin-releasing hormone secretion.; Exhibits potent and dose-dependent vasoconstrictor and anti-edema activity in the cutaneous microvasculature, a physiologic effects which does not appear to be mediated via GALR1 or GALR2. Exhibits antimicrobial activity against Gram-negative bacterias, inducing bacterial membrane blebbing.
基因功能參考文獻：
1. results indicated that obese individuals have higher plasma GAL and GALP concentrations and both peptide concentrations were positively correlative to triglyceride concentrations in obese human PMID: 27474259
2. One isoform (alarin) of this gene but not the other (galanin-like peptide) displays antimicrobial activity against E. coli. PMID: 23537644
3. This review presents considerable evidence that has accumulated implicating both galanin and GALP as playing important roles in regulating food and water intake behavior and related neuroendocrine functions. PMID: 16787232
4. describes cloning of the full-length human GALP PMID: 10601261
5. describes the gene structure of human GALP. PMID: 11861493
6. reports immunostaining of human GALP in the dermal microvasculature. PMID: 17535903
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亞細胞定位：

Secreted.
蛋白家族：

Galanin family
組織特異性：

Isoform 2 is found in ganglia of ganglioneuroma and ganglioneuroblastoma, as well as in differentiated tumor cells of neuroblastoma tissues. Not found in undifferentiated neuroblasts. Isoform 2 is found in the skin, in pericytes covering microvascular art
數據庫鏈接：

HGNC: 24840

OMIM: 611178

KEGG: hsa:85569

STRING: 9606.ENSP00000349884

UniGene: Hs.283915

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.