Human Free Thyroxine,FT4 ELISA Kit
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中文名稱：人游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E05078h
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規(guī)格：96T/48T
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價(jià)格：￥3600/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人游離甲狀腺素(FT4)酶聯(lián)免疫試劑盒（CSB-E05078h）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的FT4含量。FT4即游離甲狀腺素，是甲狀腺功能檢查的重要指標(biāo)。它能反映甲狀腺的功能狀態(tài)，不受甲狀腺結(jié)合蛋白影響。其研究機(jī)制主要圍繞甲狀腺激素的合成、分泌、調(diào)節(jié)等過(guò)程，對(duì)診斷甲亢、甲減等甲狀腺疾病具有關(guān)鍵意義。試劑盒檢測(cè)范圍為4 pmol/L-64 pmol/L，該產(chǎn)品可應(yīng)用于科研領(lǐng)域中對(duì)甲狀腺功能調(diào)控機(jī)制的研究，例如甲狀腺功能亢進(jìn)/減退動(dòng)物模型評(píng)估、環(huán)境因素對(duì)甲狀腺激素代謝的影響分析，或藥物干預(yù)實(shí)驗(yàn)中激素水平變化的動(dòng)態(tài)監(jiān)測(cè)，為內(nèi)分泌系統(tǒng)相關(guān)基礎(chǔ)研究提供可靠數(shù)據(jù)支持。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫：FT4
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma
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檢測(cè)范圍：4 pmol/L-64 pmol/L
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靈敏度：1.08 pmol/L
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Others
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human FT4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 85-99 1:2 Average % 90 Range % 87-100 1:4 Average % 95 Range % 88-98 1:8 Average % 95 Range % 86-98 -
回收率：
The recovery of human FT4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 96 91-98 EDTA plasma (n=4) 96 93-102 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pmol/L OD1 OD2 Average 4 2.547 2.646 2.597 8 1.435 1.495 1.465 16 0.797 0.851 0.824 32 0.517 0.523 0.520 64 0.307 0.295 0.301 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Characteristics and Blood Pressure Profile of Goitre Patients in A Tertiary Hospital in South-West Nigeria AO AJIBARE,ResearchGate,2021
- Prevalence and clinical relevance of thyroid autoantibodies in patients with goitre in Nigeria OA Ojo, et al,Journal of Endocrinology, Metabolism and Diabetes of South Africa,2019
- Receptor for advanced glycation end products reveals a mechanism regulating thyroid hormone secretion through the SIRT1/Nrf2 pathway Chen XJ.et al,journal of cellular biochemistry,2018
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