Human Free Testosterone,F-TESTO ELISA Kit

產品詳情

文獻引用 (7) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人游離睪酮(F-TESTO)酶聯免疫試劑盒（CSB-E05096h）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的F-TESTO含量。人游離睪酮（F-TESTO）是睪酮的一種游離形式，對男性健康和生理功能至關重要。其研究背景涉及睪酮的生理作用、代謝途徑及其與疾病如前列腺癌的關系。研究機制主要關注F-TESTO在體內的生物活性、代謝過程及其對健康的影響。試劑盒檢測范圍為7.5 pg/mL-1500 pg/mL，適用于精準分析低濃度樣本。適用于科研場景下探究內分泌失調、多囊卵巢綜合征、代謝綜合征等疾病的分子機制，或評估運動生理學、衰老相關激素水平變化等研究。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

F-TESTO
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

7.5 pg/mL-1500 pg/mL
靈敏度：

3.75 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human F-TESTO in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-102
1:2	Average %	94
	Range %	91-98
1:4	Average %	97
	Range %	93-101
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of human F-TESTO spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	92-104
EDTA plasma (n=4)	105	101-109