Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

Human D-Lactate Dehydrogenase,D-LDH ELISA Kit

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文獻引用 (2) 說明書下載

中文名稱：

人D-乳酸脫氫酶(D-LDH)酶聯免疫試劑盒
貨號：

CSB-E11720h
規格：

96T/48T
價格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人D-乳酸脫氫酶(D-LDH)酶聯免疫試劑盒（CSB-E11720h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的LDHD含量。人D-乳酸脫氫酶（D-LDH）在腫瘤代謝中起關鍵作用，其研究機制涉及在厭氧條件下催化D-乳酸生成丙酮酸，影響腫瘤生長和代謝。D-LDH基因敲除可提高D-乳酸產量，純度高達99%以上，為D-乳酸工業化生產提供新途徑。試劑盒檢測范圍為31.25 mIU/mL-2000 mIU/mL，該產品可廣泛應用于腸道功能研究、代謝通路探索、疾病模型構建等方向，例如評估缺血再灌注損傷、腸道屏障功能障礙或微生物代謝研究中D - LDH的動態變化，為能量代謝調控機制及病理生理研究提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

D lactate dehydrogenase ELISA Kit; DLD ELISA Kit; Lactate dehydrogenase D ELISA Kit; Ldhd ELISA Kit; LDHD_HUMAN ELISA Kit; MGC57726 ELISA Kit; mitochondrial ELISA Kit; Probable D lactate dehydrogenase mitochondrial ELISA Kit; Probable D-lactate dehydrogenase ELISA Kit
縮寫：

LDHD
Uniprot No.：

Q86WU2
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 mIU/mL-2000 mIU/mL
靈敏度：

7.8 mIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human D-LDH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	87-103
1:2	Average %	91
	Range %	86-103
1:4	Average %	94
	Range %	86-105
1:8	Average %	91
	Range %	84-100

回收率：

The recovery of human D-LDH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	91-106
EDTA plasma (n=4)	96	86-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average	Corrected
2000	2.418	2.385	2.402	2.265
1000	1.773	1.728	1.751	1.614
500	0.936	1.002	0.969	0.832
250	0.624	0.651	0.638	0.501
125	0.407	0.422	0.415	0.278
62.5	0.282	0.287	0.285	0.148
31.25	0.253	0.264	0.259	0.122
0	0.135	0.139	0.137

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

PPARA ameliorates sepsis-induced myocardial injury via promoting macrophage M2 polarization by interacting with DUSP1 L Cheng,Regenerative therapy,2024
Hepatocellular toxicity of oxalicumone A via oxidative stress injury and mitochondrial dysfunction in healthy human liver cells Shi S.et al,Mol Med Rep,2017

功能：

Involved in D-lactate, but not L-lactate catabolic process.
基因功能參考文獻：
1. The activity and expression of the mitochondrial D-lactate dehydrogenase, is higher in prostate cancer versus normal prostate cells. PMID: 23333299
2. Elevated serum LDH isoenzymes and AST indicate a disturbance (of uncertain clinical significance) within multiple extraosseous tissues when there is CLCN7 deficiency. PMID: 20499337
3. identification and characterization of human and mouse D-lactate dehydrogenase (NAD) [D-LDH] PMID: 12127981
亞細胞定位：

Mitochondrion.
蛋白家族：

FAD-binding oxidoreductase/transferase type 4 family
組織特異性：

Expressed moderately in heart and liver and at lower levels in skeletal muscle and kidney.
數據庫鏈接：

HGNC: 19708

OMIM: 607490

KEGG: hsa:197257

STRING: 9606.ENSP00000300051

UniGene: Hs.380929

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.