Human Cytokine-like protein 1(CYTL1) ELISA kit

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中文名稱：

人細胞因子樣蛋白1(CYTL1)酶聯免疫試劑盒
貨號：

CSB-EL006472HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人細胞因子樣蛋白1(CYTL1)酶聯免疫試劑盒（CSB-EL006472HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYTL1含量。CYTL1是一種小分子量的分泌蛋白，最初在CD34+細胞中發現。研究發現，CYTL1在細胞內具有新型抑癌因子特征，通過穩定NDUFV1抑制乳腺癌代謝重編程，其作用機制涉及與MDM2競爭性結合在NDUFV1的1-80結構域，從而干擾NDUFV1的泛素化降解，實現穩態的OXPHOS代謝。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，可廣泛應用于細胞因子調控機制研究、疾病動物模型中的蛋白表達動態分析，或體外培養細胞上清液中CYTL1分泌水平的定量評估，為探索其生物學功能及信號通路提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

C17 ELISA Kit; C4orf4 ELISA Kit; CYTL1 ELISA Kit; CYTL1_HUMAN ELISA Kit; Cytokine like 1 ELISA Kit; Cytokine like protein C17 ELISA Kit; Cytokine-like protein 1 ELISA Kit; Protein C17 ELISA Kit
縮寫：

CYTL1
Uniprot No.：

Q9NRR1
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CYTL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	82-91
1:2	Average %	94
	Range %	89-98
1:4	Average %	94
	Range %	90-97
1:8	Average %	92
	Range %	88-97

回收率：

The recovery of human CYTL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	103	98-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.008	2.102	2.055	1.877
5	1.735	1.797	1.766	1.588
2.5	1.408	1.369	1.389	1.211
1.25	1.121	1.068	1.095	0.917
0.625	0.737	0.728	0.733	0.555
0.312	0.488	0.468	0.478	0.300
0.156	0.336	0.331	0.334	0.156
0	0.178	0.178	0.178

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

引用文獻

Intracellular CYTL1, a novel tumor suppressor, stabilizes NDUFV1 to inhibit metabolic reprogramming in breast cancer W Xue,Signal Transduction and Targeted Therapy,2022
Interleukin-35 regulates peripheral T cell activity in patients with Kawasaki disease M Sun,International Immunopharmacology,2021

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基因功能參考文獻：
1. Circular dichroism analysis showed that, like chemokines, CYTL1has a higher content of beta-sheet than alpha-helix secondary structure; recombinant CYTL1 promoted calcium flux in chondrocytes; unlike chemokines, CYTL1 had limited affinity to proteoglycans; together, these properties further support cytokine-like properties for CYTL1 with some overlap with the chemokines PMID: 28478073
2. this study shows that CYTL1 possesses chemotactic activity and demonstrates that its functional receptor is CCR2B PMID: 27084102
3. when the native signal peptide of CYTL1 was replaced by IgGkappaSP, the expression and secretion was increased by 4 fold. PMID: 26922322
4. role in regulation embryo implantation PMID: 26800213
5. Cytl1 is highly expressed in the endometrium during the embryo implantation and its expression is regulated by ovarian hormones. Cytl1 enhances endometrial proliferation, induces the secretion of endometrial LIF and HB-EGF, and even enhances endometrial cell adhesion to trophoblastic cells. These indicate that Cytl1 is a potential molecular mediator of embryo is a potential molecular mediator in embryo implantation. PMID: 26800213
6. our results showed the first evidence of CYTL1 expression in SH-SY5Y neuroblastoma cells and human NB tissues, revealed a possible link between CYTL1 and NB development PMID: 22797702
7. evidence suggests that ANKRD7 and CYTL1 genes may play an important role in the variance in alcohol drinking risk PMID: 22613542
8. C17orf62-L could induce cell death accompanied with rising of cleaved PARP. PMID: 21503106
9. The DNA methylation pattern of the CYTL1 promoter region was significantly different between early and advanced stages of squamous cell carcinoma. PMID: 22011669
10. C17 as a cytokine that s contributes to immune homeostasis systemically or in a tissue-specific manner in the joint PMID: 21799806
11. We report a structural-functional characterization of cytokine-like protein 1 (Cytl1) by a combination of different computational structure-based techniques. PMID: 21322034
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亞細胞定位：

Secreted.
組織特異性：

Specifically expressed in CD34+ hematopoietic cells.
數據庫鏈接：

HGNC: 24435

OMIM: 607930

KEGG: hsa:54360

STRING: 9606.ENSP00000303550

UniGene: Hs.13872

Human Cytokine-like protein 1(CYTL1) ELISA kit

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