Human Complement fragment 4b,C4b ELISA Kit

產品詳情

文獻引用 (5) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人補體片斷4b(C4b)酶聯免疫試劑盒（CSB-E09302h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的C4b含量。C4b是補體系統經典途徑和凝集素途徑激活過程產生的重要片段。補體系統在免疫防御等方面發揮關鍵作用。研究顯示，C4b可參與調理吞噬、免疫黏附等過程，其異常活動可能與多種免疫相關疾病發生發展有關，是免疫學研究重要靶點。試劑盒檢測范圍為15.6 ng/mL-1000 ng/mL，適用于科研領域中對補體系統功能的探索，例如在炎癥模型、免疫調節實驗或疾病機制研究中定量分析血清/血漿樣本的C4b表達動態，為揭示補體介導的生理病理過程提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

Basic complement C4 ELISA Kit; C2B5 ELISA Kit; C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3 ELISA Kit; C4b ELISA Kit; C4B1 ELISA Kit; C4B2 ELISA Kit; C4B3 ELISA Kit; CO4B_HUMAN ELISA Kit; Complement C4 gamma chain ELISA Kit; Complement component 4B ELISA Kit; CPAMD3 ELISA Kit
縮寫：

C4b
Uniprot No.：

P0C0L5
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

15.6 ng/mL-1000 ng/mL
靈敏度：

3.9 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human C4b in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:200	Average %	84
	Range %	80-94
1:400	Average %	97
	Range %	91-102
1:800	Average %	98
	Range %	92-105
1:1600	Average %	92
	Range %	86-99

回收率：

The recovery of human C4b spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-98
EDTA plasma (n=4)	98	90-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.; Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
相關疾?。?/div>
Systemic lupus erythematosus (SLE); Complement component 4B deficiency (C4BD)
亞細胞定位：

Secreted. Cell junction, synapse. Cell projection, axon. Cell projection, dendrite.
組織特異性：

Complement component C4 is expressed at highest levels in the liver, at moderate levels in the adrenal cortex, adrenal medulla, thyroid gland, and the kidney, and at lowest levels in the heart, ovary, small intestine, thymus, pancreas and spleen. The extr
數據庫鏈接：

HGNC: 1324

OMIM: 120820

KEGG: hsa:100293534

STRING: 9606.ENSP00000415941

UniGene: Hs.534847