Human Coenzyme Q10 (CoQ10) ELISA Kit

產品詳情

文獻引用 (9) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

人輔酶Q10 (CoQ10)酶聯免疫試劑盒（CSB-E14081h）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CoQ10含量。CoQ10即輔酶Q10，是一種存在于人體細胞內的脂溶性醌類化合物，參與細胞能量生成與抗氧化防御。研究機制方面，它在線粒體呼吸鏈中傳遞電子、質子，促進ATP合成，還能清除自由基，保護細胞免受氧化損傷。試劑盒檢測范圍為3.12 ng/mL-50 ng/mL，支持能量代謝研究、疾病模型機制探索（如心肌缺血、帕金森病等）以及膳食補充劑效果評估等實驗需求本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

N/A
縮寫：

CoQ10
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-50 ng/mL
靈敏度：

1.56 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CoQ10 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	96
	Range %	91-101
1:40	Average %	99
	Range %	93-104
1:80	Average %	92
	Range %	87-96
1:160	Average %	90
	Range %	85-94

回收率：

The recovery of human CoQ10 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	80-88
EDTA plasma (n=4)	93	88-97