Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

Human Carboxypeptidase N subunit 2(CPN2) ELISA kit

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中文名稱：

人羧基肽酶N亞基2(CPN2)酶聯免疫試劑盒
貨號：

CSB-EL005899HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人羧基肽酶N亞基2(CPN2)酶聯免疫試劑盒（CSB-EL005899HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CPN2含量。人羧基肽酶N亞基2（CPN2）是一種羧肽酶，參與蛋白質降解和細胞信號傳導。其研究機制涉及CPN2在蛋白質降解過程中的作用，以及如何通過調節細胞內信號通路影響細胞功能。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，適用于基礎科研中探索CPN2在心血管疾病、免疫調節或炎癥機制中的生物學功能，亦可用于體外模型中蛋白表達水平的動態監測。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

CPN2 ELISA kit; ACBPCarboxypeptidase N subunit 2 ELISA kit; Carboxypeptidase N 83 kDa chain ELISA kit; Carboxypeptidase N large subunit ELISA kit; Carboxypeptidase N polypeptide 2 ELISA kit; Carboxypeptidase N regulatory subunit ELISA kit
縮寫：

CPN2
Uniprot No.：

P22792
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.156 ng/mL-10 ng/mL
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CPN2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	99
	Range %	92-105
1:2	Average %	106
	Range %	102-112
1:4	Average %	97
	Range %	91-103
1:8	Average %	93
	Range %	89-98

回收率：

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

2.858

2.934

2.896

2.755

2.131

2.167

2.149

2.008

2.5

1.275

1.328

1.302

1.160

1.25

0.837

0.829

0.833

0.692

0.625

0.492

0.521

0.506

0.365

0.312

0.317

0.323

0.320

0.179

0.156

0.238

0.244

0.241

0.100

0.137

0.145

0.141

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

The 83 kDa subunit binds and stabilizes the catalytic subunit at 37 degrees Celsius and keeps it in circulation. Under some circumstances it may be an allosteric modifier of the catalytic subunit.
基因功能參考文獻：
1. Real-time PCR and western blotting show the following CPN2 expression: minimal in MDA-MB-468 cells but obvious in MDA-MB-231 and MCF7 cells. MDA-MB-231 breast cancer cell lines were selected for the control group and CPN2 was knocked out for the experimental group. PMID: 26860443
亞細胞定位：

Secreted.
數據庫鏈接：

HGNC: 2313

OMIM: 603104

KEGG: hsa:1370

STRING: 9606.ENSP00000319464

UniGene: Hs.528368

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.