Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

說明書下載

中文名稱：

人羧基肽酶N催化鏈(CPN1)酶聯免疫試劑盒
貨號：

CSB-EL005898HU
規格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

人羧基肽酶N催化鏈(CPN1)酶聯免疫試劑盒（CSB-EL005898HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CPN1含量。CPN1是一種細胞分裂素/嘌呤核糖核苷核苷酶，參與植物生長促進激素細胞分裂素的代謝。研究發現，CPN1存在于細胞壁空間中，通過活化從根部運輸的前體，影響水稻穗形成等生長過程，有望應用于提高作物產量。試劑盒檢測范圍為31.25 mU/mL-2000 mU/mL，適用于體外實驗中探索CPN1在炎癥反應調控、血管功能異常或代謝性疾病中的分子機制，也可用于評估不同實驗條件下（如藥物處理或基因干預）生物樣本中CPN1的活性動態變化。該產品為研究CPN1在基礎科研中的生物學功能及其作為潛在生物標志物的應用提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

ACBP ELISA Kit; Anaphylatoxin inactivator ELISA Kit; Arginine carboxypeptidase ELISA Kit; Carboxypeptidase N catalytic chain ELISA Kit; Carboxypeptidase N polypeptide 1 50 kD ELISA Kit; Carboxypeptidase N polypeptide 1 ELISA Kit; Carboxypeptidase N small subunit ELISA Kit; Carboxypeptidase N subunit 1 ELISA Kit; CBPN_HUMAN ELISA Kit; CPN ELISA Kit; CPN1 ELISA Kit; Kininase 1 ELISA Kit; Kininase-1 ELISA Kit; Kininase1 ELISA Kit; Lysine carboxypeptidase ELISA Kit; Plasma carboxypeptidase B ELISA Kit; SCPN ELISA Kit; Serum carboxypeptidase N ELISA Kit
縮寫：

CPN1
Uniprot No.：

P15169
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 mU/mL-2000 mU/mL
靈敏度：

7.8 mU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CPN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	85-95
1:2	Average %	105
	Range %	101-109
1:4	Average %	87
	Range %	84-92
1:8	Average %	95
	Range %	91-99

回收率：

The recovery of human CPN1 piked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	93-103
EDTA plasma (n=4)	92	85-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mU/ml	OD1	OD2	Average	Corrected
2000	1.998	2.112	2.055	1.916
1000	1.547	1.675	1.611	1.472
500	1.122	1.132	1.127	0.988
250	0.683	0.688	0.686	0.547
125	0.436	0.454	0.445	0.306
62.5	0.321	0.325	0.323	0.184
31.25	0.232	0.241	0.237	0.098
0	0.138	0.140	0.139

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

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功能：

Protects the body from potent vasoactive and inflammatory peptides containing C-terminal Arg or Lys (such as kinins or anaphylatoxins) which are released into the circulation.
基因功能參考文獻：
1. CPN binds to fibrinogen and is present in a fibrin clot prepared from plasma. PMID: 23000409
2. carboxypeptidase N regulates the biologic activity of SDF-1alpha by reducing the chemokine-specific activity PMID: 15718415
3. This review summarizes the structure, enzymatic properties and function of this enzyme, including insights gained by the recent elucidation of the crystal structure of the CPN catalytic subunit and structural modeling of the non-catalytic 83 kDa subunit. PMID: 18039526
相關疾病：

Carboxypeptidase N deficiency (CPND)
亞細胞定位：

Secreted, extracellular space.
蛋白家族：

Peptidase M14 family
組織特異性：

Synthesized in the liver and secreted in plasma.
數據庫鏈接：

HGNC: 2312

OMIM: 212070

KEGG: hsa:1369

STRING: 9606.ENSP00000359446

UniGene: Hs.2246

Human Carboxypeptidase N catalytic chain(CPN1) ELISA kit

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