Human Calponin-1(CNN1)ELISA Kit

Human Calponin-1(CNN1)ELISA Kit

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中文名稱：

人調(diào)寧蛋白- 1(CNN1)酶聯(lián)免疫試劑盒
貨號：

CSB-E14109h
規(guī)格：

96T/48T
價格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

人調(diào)寧蛋白- 1(CNN1)酶聯(lián)免疫試劑盒（CSB-E14109h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的CNN1含量。試劑盒檢測范圍為0.156 ng/mL-10 ng/mL，本產(chǎn)品適用于基礎醫(yī)學研究、藥效評估及疾病機制探索等科研場景，為心血管病理研究、抗纖維化藥物開發(fā)及腫瘤微環(huán)境分析提供可靠檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

Basic calponin ELISA Kit; Calponin 1 ELISA Kit; Calponin 1 basic smooth muscle ELISA Kit; Calponin H1 ELISA Kit; Calponin H1 smooth muscle ELISA Kit; Calponin-1 ELISA Kit; Calponin1 ELISA Kit; Calponins basic ELISA Kit; CNN 1 ELISA Kit; Cnn1 ELISA Kit; CNN1_HUMAN ELISA Kit; Epididymis secretory protein Li 14 ELISA Kit; HEL S 14 ELISA Kit; Sm Calp ELISA Kit; SMCC ELISA Kit; smooth muscle ELISA Kit
縮寫：

CNN1
Uniprot No.：

P51911
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

0.156 ng/ml-10 ng/ml
靈敏度：

0.039 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Human CNN1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	87
	Range %	84-92
1:2	Average %	95
	Range %	90-99
1:4	Average %	94
	Range %	85-99
1:8	Average %	104
	Range %	98-108

回收率：

The recovery of Human CNN1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	87	83-94

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.255	2.345	2.300	2.202
5	1.715	1.756	1.736	1.638
2.5	1.064	1.024	1.044	0.946
1.25	0.523	0.515	0.519	0.421
0.625	0.334	0.324	0.329	0.231
0.312	0.251	0.234	0.243	0.145
0.156	0.162	0.159	0.161	0.063
0	0.097	0.098	0.098

數(shù)據(jù)處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Thin filament-associated protein that is implicated in the regulation and modulation of smooth muscle contraction. It is capable of binding to actin, calmodulin and tropomyosin. The interaction of calponin with actin inhibits the actomyosin Mg-ATPase activity.
基因功能參考文獻：
1. Studied maternal serum calponin 1 levels in the prediction of delivery within 7 days among pregnancies complicated with threatened preterm labor. Of 73 women included in the study, found the maternal serum calponin 1 level was significantly high in women who delivered within 7 days vs. those who delivered after 7 days. PMID: 28068849
2. Loss of myoepithelial calponin was specifically associated with gain of the basal marker p63 in adjacent tumor cells. PMID: 26343330
3. Loss of CNN1 expression is associated with Ovarian Cancer. PMID: 26504022
4. CNN1 immunohistochemical staining allows ready recognition of intracytoplasmic inclusions, aiding diagnosis of infantile digital fibromatosis. PMID: 24117680
5. epsilonPKC/ERK/mTOR pathway activation induced by the rescued expression of CNN1 contributed to the improvement of cardiac dysfunction and pathological changes observed in transgenic mice. PMID: 24631115
6. Calponin knockdown inhibits autophosphorylation. PMID: 24350264
7. Expression of transforming growth factor-beta (4.3 +/- 1.4 vs 2.6 +/- 2.3, p = 0.01) and calponin (3.7 +/- 0.7 vs 0.6 +/- 1.1, p = 0.05) was lower from infants with vs without a PDA. PMID: 23731614
8. Calponin-1 expression in the uterine smooth muscle tissue of the body and the lower uterine segment of smooth muscle tissues was significantly different. PMID: 21051832
9. Inhibition of calponin-1 expression can inhibit uterine smooth muscle cell migration and cause the morphological change and rearrangement of F-actin without affecting its proliferation and apoptosis in vitro. PMID: 20584679
10. A single intronic CArG element is necessary but insufficient for proper CNN1 expression in vivo. CNN1 overexpression antagonizes arterial injury-induced neointimal formation. PMID: 21817093
11. D2-40 immunohistochemistry reliably identifies the myoepithelial cells of breast in a variety of lesions in a pattern similar to that of calponin and p63. PMID: 21326813
12. The point mutation S175T exhibited more resistance to actin rearrangement by cytochalasin D and PDBu than intact CNh1, suppressing cell motility induced by PDBu. PMID: 20530411
13. expression in tumor vessels is a new and useful means to predict prognosis of hepatocellular carcinoma after hepatic resection PMID: 11920541
14. results suggest that regulation of h1-calponin is involved in the regulation of differentiation of AR42J cells into insulin-producing cells PMID: 12606518
15. the calponin homology domain of Vav1 associates with calmodulin and is prerequisite to T cell antigen receptor-induced calcium release in Jurkat T lymphocytes PMID: 17550897
16. analysis of distinct sites of interaction that form the calponin: gelsolin complex and two calcium switches that control its activity PMID: 17556051
17. expression of vascular CNN1 in the peripheral region of colon cancer tissues was significantly reduced in association with tumor progression, lymphatic invasion, vascular invasion and recurrence. PMID: 17707120
18. Calponin and alpha-inhibin are the most useful positive markers for serous microcystic adenoma, and are negative in most entities in the differential diagnosis. PMID: 19391217
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蛋白家族：

Calponin family
組織特異性：

Smooth muscle, and tissues containing significant amounts of smooth muscle.
數(shù)據(jù)庫鏈接：

HGNC: 2155

OMIM: 600806

KEGG: hsa:1264

STRING: 9606.ENSP00000252456

UniGene: Hs.465929

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.