Human C-type lectin domain family 9 member A(CLEC9A) ELISA kit

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中文名稱：

人C型凝集素結(jié)構(gòu)域家族9成員A(CLEC9A)酶聯(lián)免疫試劑盒
貨號：

CSB-EL005542HU
規(guī)格：

96T/48T
價(jià)格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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小鼠C型凝集素結(jié)構(gòu)域家族9成員A(CLEC9A)酶聯(lián)免疫試劑盒（CSB-EL005542HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細(xì)胞裂解物樣本中的CLEC9A含量。CLEC9A是一個(gè)具有研究價(jià)值的靶點(diǎn)。它在免疫系統(tǒng)中發(fā)揮作用，能識(shí)別受損細(xì)胞釋放的特定分子模式。相關(guān)研究致力于揭示其信號傳導(dǎo)機(jī)制，探索它在免疫激活、抗腫瘤免疫等方面的潛力，有望為開發(fā)免疫治療新策略提供方向。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于科研場景下探究CLEC9A在免疫調(diào)控、炎癥反應(yīng)或病原體感染中的分子機(jī)制，亦可作為體外實(shí)驗(yàn)中的生物標(biāo)志物檢測工具，支持通過多種生物樣本（如血清中的可溶性蛋白或組織微環(huán)境中的表達(dá)水平）進(jìn)行定量分析，為免疫學(xué)、細(xì)胞生物學(xué)相關(guān)研究提供標(biāo)準(zhǔn)化檢測方案。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

C type lectin domain family 9; member A ELISA Kit; C-type lectin domain family 9 member A ELISA Kit; CLC9A_HUMAN ELISA Kit; CLEC9A ELISA Kit; DNGR1 ELISA Kit; HEEE9341 ELISA Kit; PRO34046 ELISA Kit; UNQ9341 ELISA Kit
縮寫：

CLEC9A
Uniprot No.：

Q6UXN8
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

0.312 ng/mL-20 ng/mL
靈敏度：

0.078 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human CLEC9A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-93
1:2	Average %	104
	Range %	100-108
1:4	Average %	89
	Range %	85-93
1:8	Average %	108
	Range %	104-112

回收率：

The recovery of human CLEC9A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-93
EDTA plasma (n=4)	106	103-109

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.273	2.109	2.191	2.038
10	1.575	1.604	1.590	1.437
5	1.179	1.205	1.192	1.039
2.5	0.825	0.825	0.825	0.672
1.25	0.525	0.532	0.529	0.376
0.625	0.375	0.368	0.372	0.219
0.312	0.264	0.261	0.263	0.110
0	0.154	0.152	0.153

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

引用文獻(xiàn)

Increased expression of Clec9A on cDC1s associated with cytotoxic CD8+ T cell response in COPD L Yan,Clinical immunology,2022
The Inflammatory Factors Associated with Disease Severity to Predict COVID-19 Progression W Huang,The Journal of Immunology,2021
Reduced CLEC9A expression in synovial tissue of psoriatic arthritis patients after adalimumab therapy. Ramos MI.et al,Rheumatology (Oxford).,2016

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功能：

Functions as an endocytic receptor on a small subset of myeloid cells specialized for the uptake and processing of material from dead cells. Recognizes filamentous form of actin in association with particular actin-binding domains of cytoskeletal proteins, including spectrin, exposed when cell membranes are damaged, and mediate the cross-presentation of dead-cell associated antigens in a Syk-dependent manner.
基因功能參考文獻(xiàn)：
1. Clec9a is responsible for the antigen cross-presentation of dendritic cell subsets and may be a target of immunotherapy PMID: 27250027
2. findings demonstrate that CLEC9A is a specialized receptor that modulates the innate immune response when there is a mycobacterial infection PMID: 29065139
3. Data indicate that blood dendritic cell antigen 3 BDCA3(+) and C-type lectin domain family 9, member A CLEC9A(+) dendritic cells (DC) are of major importance in the induction of anti-viral and anti-tumor immunity. PMID: 24910448
4. Activated dendritic cell subsets expressing CD141/CLEA9A/CD1c, likely recruited into the tubulointerstitium, are positioned to play a role in the development of fibrosis and, thus, progression to chronic kidney disease. PMID: 24049150
5. study showsn that the DNGR-1 ligand is preserved from yeast to man and corresponds to the F-actin component of the cellular cytoskeleton; identification of F-actin as a DNGR-1 ligand suggests that cytoskeletal exposure is a universal sign of cell damage that can be targeted by the innate immune system to initiate immunity PMID: 22483800
6. High expression of DNGR-1 specifically and universally identifies a unique dendritic cells subset in mouse and humans. PMID: 22442345
7. propose that Clec9A provides targeted recruitment of the adaptive immune system during infection and can also be utilized to enhance immune responses generated by vaccines. PMID: 22483802
8. Data show that CLEC9A is only expressed on immature BDCA3(+) myeloid dendritic cells (mDCs). PMID: 22234694
9. For production of cytotoxic T cells, transgenic DEC-205 and Clec9A, but not Clec12A, are effective targets for antibody-mediated delivery of antigens for vaccination, although only in the presence of adjuvants. PMID: 21677141
10. A population of human dendritic cells (DC) that expresses DNGR-1 (CLEC9A) and high levels of BDCA3 and resembles mouse CD8alpha+ DCs in phenotype and function, is characterized. PMID: 20479117
11. DC, NK lectin group receptor-1 (DNGR-1) is a C-type lectin of the NK cell receptor group PMID: 18497879
12. CLEC9A functions as an activation receptor PMID: 18408006
13. analysis of differences between mouse and human Clec9A PMID: 18669894
14. CLEC9A functions as a SYK-coupled C-type lectin receptor to mediate sensing of necrosis by the principal dendritic-cell subset involved in regulating cross-priming to cell-associated antigens PMID: 19219027
15. The paper cited (J Clin Invest. 2008 Jun;118(6):2098-110) is the first characterization of human and mouse CLEC9A (a.k.a DNGR-1) PMID: 18497879
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亞細(xì)胞定位：

Membrane; Single-pass type II membrane protein.
組織特異性：

In peripheral blood highly restricted on the surface of BDCA31(+) dendritic cells and on a small subset of CD14(+) and CD16(-) monocytes.
數(shù)據(jù)庫鏈接：

HGNC: 26705

OMIM: 612252

KEGG: hsa:283420

STRING: 9606.ENSP00000348074

UniGene: Hs.531189

Human C-type lectin domain family 9 member A(CLEC9A) ELISA kit

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