Human Aquaporin 4 Antibody,AQP-4 Ab ELISA Kit

產(chǎn)品詳情

文獻引用 (1) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

人水通道蛋白4抗體(AQP-4 Ab)酶聯(lián)免疫試劑盒（CSB-E13568h）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿樣本中的AQP-4 Ab 含量。AQP - 4 Ab是一種靶點。AQP - 4即水通道蛋白4，其抗體（Ab）在神經(jīng)系統(tǒng)疾病尤其視神經(jīng)脊髓炎譜系疾病中意義重大。研究機制上，它可結(jié)合AQP - 4，介導補體激活、免疫細胞浸潤等免疫病理過程，損傷神經(jīng)組織。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研場景中疾病模型構(gòu)建、抗體表達水平研究、藥物干預效果評估等實驗需求；為探索 AQP - 4 抗體在神經(jīng)免疫疾病中的分子機制、篩選潛在治療靶點等基礎研究提供可靠工具，檢測數(shù)據(jù)可服務于細胞實驗、動物模型研究及生物標志物分析等方向。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

AQP-4 Ab
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human AQP-4 antibody in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-95
1:2	Average %	94
	Range %	84-105
1:4	Average %	97
	Range %	92-101
1:8	Average %	91
	Range %	82-97

回收率：

The recovery of human AQP-4 antibody spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	82-197
EDTA plasma (n=4)	95	84-105

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.