Human Alpha-enolase (ENO1/ENO1L1/MBPB1/MPB1) ELISA kit
產(chǎn)品詳情
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產(chǎn)品描述：
人α-烯醇化酶(ENO1/ENO1L1/MBPB1/MPB1)酶聯(lián)免疫試劑盒（CSB-E17177h）為雙抗夾心法ELISA試劑盒，定量檢測血清、組織勻漿、血漿樣本中的ENO1含量。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于生命科學(xué)領(lǐng)域研究，如疾病分子機制探索、細胞代謝調(diào)控分析、腫瘤微環(huán)境研究等方向，為科研人員提供穩(wěn)定可靠的檢測工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：
2 phospho D glycerate hydro lyase ELISA Kit; 2-phospho-D-glycerate hydro-lyase ELISA Kit; Alpha enolase ELISA Kit; Alpha enolase like 1 ELISA Kit; Alpha-enolase ELISA Kit; C myc promoter binding protein ELISA Kit; C-myc promoter-binding protein ELISA Kit; EC 4.2.1.11 ELISA Kit; eno1 ELISA Kit; ENO1L1 ELISA Kit; ENOA_HUMAN ELISA Kit; Enolase 1 (alpha) ELISA Kit; Enolase 1 (alpha) like 1 ELISA Kit; Enolase 1 ELISA Kit; Enolase alpha ELISA Kit; MBP 1 ELISA Kit; MBP-1 ELISA Kit; MBP1 ELISA Kit; MBPB1 ELISA Kit; MPB 1 ELISA Kit; MPB-1 ELISA Kit; MPB1 ELISA Kit; MYC promoter binding protein 1 ELISA Kit; NNE ELISA Kit; Non neural enolase ELISA Kit; Non-neural enolase ELISA Kit; Phosphopyruvate hydratase ELISA Kit; Plasminogen binding protein ELISA Kit; Plasminogen-binding protein ELISA Kit; PPH ELISA Kit; Tau crystallin ELISA Kit
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縮寫：
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Uniprot No.：
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種屬：
Homo sapiens (Human)
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樣本類型：
serum, tissue homogenates, plasma
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檢測范圍：
0.312 ng/mL-20 ng/mL
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靈敏度：
0.078 ng/mL
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反應(yīng)時間：
1-5h
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樣本體積：
50-100ul
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檢測波長：
450 nm
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研究領(lǐng)域：
Metabolism
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測定原理：
quantitative
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測定方法：
Sandwich
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精密度：
| Intra-assay Precision (Precision within an assay): CV%<8% | | | |
| Three samples of known concentration were tested twenty times on one plate to assess. | |
| Inter-assay Precision (Precision between assays): CV%<10% | | | |
| Three samples of known concentration were tested in twenty assays to assess. | | |
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線性度：
| To assess the linearity of the assay, samples were spiked with high concentrations of human ENO1/ENO1L1/MBPB1/MPB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| | Sample | Serum(n=4) | |
| 1:1 | Average % | 94 | |
| Range % | 90-97 | |
| 1:2 | Average % | 92 | |
| Range % | 88-96 | |
| 1:4 | Average % | 94 | |
| Range % | 89-98 | |
| 1:8 | Average % | 100 | |
| Range % | 96-105 | |
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回收率：
| The recovery of human ENO1/ENO1L1/MBPB1/MPB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
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| Sample Type | Average % Recovery | Range | |
| Serum (n=5) | 94 | 89-100 | |
| EDTA plasma (n=4) | 96 | 90-102 | |
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標準曲線：
| These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
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 | ng/ml | OD1 | OD2 | Average | Corrected | | | 20 | 2.321 | 2.319 | 2.320 | 2.225 | | | 10 | 1.982 | 1.967 | 1.975 | 1.880 | | | 5 | 1.470 | 1.576 | 1.523 | 1.428 | | | 2.5 | 1.003 | 0.995 | 0.999 | 0.904 | | | 1.25 | 0.638 | 0.652 | 0.645 | 0.550 | | | 0.625 | 0.380 | 0.391 | 0.386 | 0.291 | | | 0.312 | 0.293 | 0.299 | 0.296 | 0.201 | | | 0 | 0.093 | 0.097 | 0.095 | | |
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本試劑盒所含材料：
- A micro ELISA plate ---The 96-well plate has been pre-coated with an anti-human ENO-1 antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
- Two vials lyophilized standard ---Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
- One vial Biotin-labled ENO-1 antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
- One vial HRP-avidin (100 x concentrate) (120 μl/bottle) ---Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
- One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
- One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
- One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
- One vial Wash Buffer (25 x concentrate) (20 ml/bottle) ---Wash away unbound or free substances.
- One vial TMB Substrate (10 ml/bottle) ---Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
- One vial Stop Solution (10 ml/bottle) ---Stop the color reaction. The solution color immediately turns from blue to yellow.
- Four Adhesive Strips (For 96 wells) ---Cover the microplate when incubation.
- An instruction manual
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本試劑盒不含材料：
- A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
- An incubator can provide stable incubation conditions up to 37°C±5°C.
- Centrifuge
- Vortex
- Squirt bottle, manifold dispenser, or automated microplate washer
- Absorbent paper for blotting the microtiter plate
- 50-300ul multi-channel micropipette
- Pipette tips
- Single-channel micropipette with different ranges
- 100ml and 500ml graduated cylinders
- Deionized or distilled water
- Timer
- Test tubes for dilution
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數(shù)據(jù)處理：
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貨期：
3-5 working days
引用文獻
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ENO1 Promotes OSCC Migration and Invasion by Orchestrating IL-6 Secretion from Macrophages via a Positive Feedback Loop
Y Lin,International journal of molecular sciences,2023
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ENO1 Binds to ApoC3 and Impairs the Proliferation of T Cells via IL-8/STAT3 Pathway in OSCC
J Wang,International journal of molecular sciences,2022
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Overexpressed Proteins in HCC Cell-Derived Exosomes, CCT8, and Cofilin-1 Are Potential Biomarkers for Patients with HCC
HJ Cho,Diagnostics,2021
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Piper sarmentosum Roxb. confers neuroprotection on beta-amyloid (A尾)-induced microglia-mediated neuroinflammation and attenuates tau
Yeo ETY.et al,J Ethnopharmacol,2018
靶點詳情
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功能：
Glycolytic enzyme the catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate. In addition to glycolysis, involved in various processes such as growth control, hypoxia tolerance and allergic responses. May also function in the intravascular and pericellular fibrinolytic system due to its ability to serve as a receptor and activator of plasminogen on the cell surface of several cell-types such as leukocytes and neurons. Stimulates immunoglobulin production.; MBP1 binds to the myc promoter and acts as a transcriptional repressor. May be a tumor suppressor.
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基因功能參考文獻：
- Our data provide strong evidence that alpha-enolase short hairpin RNA interference vector can effectively suppress the proliferation and increase chemosensitivity of MKN45 cells, which may provide a novel gene therapy for gastric cancer. PMID: 29986635
- Elevated ENO1 could promote the proliferation of retinoblastoma cells. PMID: 29913409
- Together these findings suggest a possible mechanism of host invasion by HIV-1 through the CAWLEAQ motif of Nef-mediated regulation of ENO1 and identify a potential therapeutic target for HIV-1 entry at mucosal barriers. PMID: 29404888
- We propose that ENO1 is a useful indicator of parakeratosis and might have a potential role in cellular TJ barrier function in the epidermis PMID: 29430682
- The authors found that ENO1 expression was upregulated in the hepatitis B virus-infected liver tissues and cells. Silencing of ENO1 resulted in a significant reduction in hepatitis B virus replication, and this siRNA-mediated reaction also caused the upregulation of expression of type I interferon and downstream interferon-stimulated genes. PMID: 29080231
- serum anti-alpha-enolase antibody levels positively correlated with serum whole IgG and 24-hour urine protein and negatively correlated with serum D-dimer level in patients with systemic lupus erythematosus PMID: 28728510
- ENO1 is a crucial factor promoting neoplastic transformation exclusively in terminal respiratory unit (TRU) subtype lung adenocarcinomas (ADCs). PMID: 29090499
- Protein arginine methyltransferase 5 (PRMT5) was identified to be responsible for Eno-1 methylation. Overexpression of PRMT5 and caveolin-1 enhanced levels of membrane-bound extracellular Eno-1 and, conversely, pharmacological inhibition of PRMT5 attenuated Eno-1 cell-surface localization. PMID: 29501774
- Our results suggested that alpha-enolase level was significantly elevated in pancreatic cancer tissues, which was closely associated with pancreatic cancer progression PMID: 28824297
- ENO1 is a novel biomarker to predict drug resistance and overall prognosis in gastric cancer. PMID: 28548950
- ENO1 can elicit humoral immune response in NSCLC and its autoantibody has association with the tumorigenesis of NSCLC. Furthermore, these intriguing results suggest the possibility of autoantibody against ENO1 serving as a potential diagnostic biomarker in NSCLC and have implications for defining novel histological determinants of NSCLC. PMID: 28456790
- The study provides solid evidence that there is the interaction between GRN A and ENO1 and the interaction is responsible for the effects of GRN A on glucose uptake as well as cancer cell migration and invasion. PMID: 28415822
- Our findings showed that both S100beta and NSE levels similarly increased during CPB and immediately after CPB during sevoflurane and propofol based anesthesia. PMID: 26856295
- ENO1 promotes in pancreatic ductal adenocarcinoma (PDA) cells cell survival, migration, and metastasis through cooperation with integrins and uPAR PMID: 28086938
- ENO1 is a potent promoter of colorectal cancer genesis and metastasis at least in part though regulating AMPK/mTOR pathway. PMID: 27996156
- comparing the T cell receptor repertoire of ENO1-specific peripheral and infiltrating tumor T cells from the same patient suggests that ENO1-specific T cells, despite having a different functional profile, can recirculate from the tumor to the periphery. PMID: 27210467
- ENO1 is able to activate in vitro the CD14-dependent TLR4 pathway on monocytes involving a dual mechanism firstly pro-inflammatory and secondly anti-inflammatory. PMID: 27025255
- High ENO1 expression is associated with cancer. PMID: 26734996
- An immune-modulatory role has been proposed for surface alpha-enolase and our findings of decreased expression suggest that deficits in surface alpha-enolase merit investigation in the context of immune dysfunction during ageing and vascular disease PMID: 26432922
- Systems biology approach reveals possible evolutionarily conserved moonlighting functions for enolase PMID: 25978602
- ENO1 was demonstrated to be up-regulated by HIF-1alpha in retinal pigment epithelial cells in response to hypoxia, without influencing VEGF secretion. PMID: 26882120
- In this study, ENO1 silencing significantly reduced cell glycolysis, proliferation, migration, and invasion in vitro, as well as tumorigenesis and metastasis in vivo by modulating p85 suppression. PMID: 25951350
- alpha-Enolase increases after injury and may activate pulmonary endothelial cells and prime PMNs through plasmin activity and PAR-2 activation PMID: 25944790
- High ENO1 expression is associated with invasiveness of pancreatic cancer. PMID: 25860938
- tRK1 forms a complex with human enolases and interacts with tRK1 and human pre-lysyl-tRNA synthetase (preKARS2) PMID: 25918939
- The association of ENO1 and GPI with postthaw sperm viability and motility was confirmed using Pearson's linear correlation. ENO1 and GPI can be used as markers of human sperm freezability before starting the cryopreservation procedure. PMID: 25910678
- This study showed that ENO1 is responsible for non-small cell lung cancer proliferation and metastasis PMID: 25887760
- ENO1 induces gene expression, lactate production, cell proliferation and migration and can be negatively regulated by FBXW7 at the posttranslational level. PMID: 26097998
- alpha-enolase binds plasminogen and modulates its activation, it is plausible to speculate the association of the increase in alpha-enolase secretion by infected hepatic cells with the haemostatic dysfunction observed in dengue patients PMID: 25171719
- Data suggest that endoplasmic reticulum stress up-regulates ENO1/MBP1 expression through the AKT/PERK/eIF2a signaling axis in both breast cancer cells and non-tumor cells. PMID: 26144282
- Low ENO1 expression is associated with clear cell renal cell carcinoma. PMID: 26037892
- ENO1 is an independent prognostic factor, significantly correlated with overall survival of Non-Hodgkin's Lymphoma patients. PMID: 26024773
- STIM1/ORAI1-mediated Ca2+ influx regulates enolase-1 exteriorization in cancer cells. PMID: 25805497
- the results indicated that the combination of ENO1 and CYPA can serve as a potential molecular marker for the early detection of NPC. PMID: 24998431
- Using shotgun proteomics together with western blotting and ELISA, we identify sputum ENO1 as a biomarker candidate for lung cancer PMID: 24984566
- Our data suggest that ENO1 was upregulated by CagA protein thereby providing a novel mechanism underlying H. pylori-mediated gastric diseases. PMID: 24841372
- These data show a multiantibody composition in lupus nephritis, where IgG2 autoantibodies against alpha-enolase and annexin AI predominate in the glomerulus and can be detected in serum. PMID: 24790181
- Isotypes of anti-citrullinated fibrinogen and alpha-enolase can be found in the serum of children with juvenile idiopathic arthritis of all onset types. PMID: 25068682
- Overexpression of ENO1 is associated with glioma progression. PMID: 24650096
- Citrullination of ENO1 is associated with neoplasms. PMID: 24099319
- the differential association of ENO-1 with caveolar proteins regulates ENO-1 subcellular localization and, consequently, ENO-1-dependent cell migration and invasion. PMID: 24628430
- ENO1 overexpression can inhibit EMT in vitro by suppressing ERK1/2 phosphorylation PMID: 23676977
- surface alpha-enolase promotes extracellular matrix degradation and invasion of cancer cells and that targeting surface alpha-enolase is a promising approach to suppress tumor metastasis. PMID: 23894455
- plasma levels are significantly higher in renal cell carcinoma patients than in controls PMID: 23113677
- results indicate that Streptococcus sanguinis infection and the sera of Behcet's disease patients with active disease are inflammatory stimuli that can induce membranous alpha-enolase expression in endothelial cells PMID: 23131860
- The reduction in enolase-1 expression significantly decreases the response to hypoxia and enhances the sensitivity of the cells to radiation therapy. PMID: 23381546
- ENO1 is a passenger locus homozygously deleted as part of the 1p36 locus in glioblastoma and inhibition of ENO2 is selectively lethal to ENO1 deleted tumor cells, but not normal non-cancerous cells. PMID: 22895339
- These results extend, confirm, and generalize the evidence supporting the specificity of the anti-CEP-1 antibody-positive subgroup of patients with rheumatoid arthritis among anti-CCP antibody-positive patients with RA. PMID: 21360494
- Data show six differentially expressed proteins were identified as HSP70, PPIA and alpha-Enolase (up-regulated) S100-A9, PIMT and beta-5 tubulin (down-regulated), most of which had been shown to play a potential role in the pathogenesis of atherosclerosis. PMID: 21839816
- results indicate that C-peptide has the capacity to activate alpha-enolase through a specific interaction between E27 of the peptide and K434 of the enzyme PMID: 22577163
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亞細胞定位：
Cytoplasm. Cell membrane. Cytoplasm, myofibril, sarcomere, M line. Note=Can translocate to the plasma membrane in either the homodimeric (alpha/alpha) or heterodimeric (alpha/gamma) form. ENO1 is localized to the M line.; [Isoform MBP-1]: Nucleus.
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蛋白家族：
Enolase family
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組織特異性：
The alpha/alpha homodimer is expressed in embryo and in most adult tissues. The alpha/beta heterodimer and the beta/beta homodimer are found in striated muscle, and the alpha/gamma heterodimer and the gamma/gamma homodimer in neurons.
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數(shù)據(jù)庫鏈接：
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