Human Acylamino-acid-releasing enzyme(APEH) ELISA kit

說明書下載

產品詳情

產品描述：

人酰基氨基酸釋放酶(APEH)酶聯(lián)免疫試劑盒（CSB-EL001899HU）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿、細胞裂解物樣本中的APEH含量。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，科研人員可通過本產品研究 APEH 在細胞代謝調控、疾病發(fā)生機制中的作用，或用于藥物開發(fā)中靶點活性評估。為體外蛋白水平研究提供高效工具。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

AARE ELISA Kit; ACPH ELISA Kit; ACPH_HUMAN ELISA Kit; Acyl peptide hydrolase ELISA Kit; Acyl-peptide hydrolase ELISA Kit; Acylamino acid releasing enzyme ELISA Kit; Acylamino-acid-releasing enzyme ELISA Kit; Acylaminoacyl peptidase ELISA Kit; Acylaminoacyl-peptidase ELISA Kit; APEH ELISA Kit; APH ELISA Kit; N acylaminoacyl peptide hydrolase ELISA Kit; OPH ELISA Kit; Oxidized protein hydrolase ELISA Kit
縮寫：

APEH
Uniprot No.：

P13798
種屬：

Homo sapiens (Human)
樣本類型：

serum, plasma, tissue homogenates, cell lysates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Others
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of human APEH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	82-87
1:2	Average %	98
	Range %	94-103
1:4	Average %	88
	Range %	84-92
1:8	Average %	108
	Range %	104-112

回收率：

The recovery of human APEH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	99	95-103
EDTA plasma (n=4)	86	86-89

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

This enzyme catalyzes the hydrolysis of the N-terminal peptide bond of an N-acetylated peptide to generate an N-acetylated amino acid and a peptide with a free N-terminus. It preferentially cleaves off Ac-Ala, Ac-Met and Ac-Ser.
基因功能參考文獻：
1. Although APEH is primarily localized in the cytoplasm, a sub-fraction of this enzyme is sequestered at sites of nuclear damage following UVA irradiation or following oxidative stress. APEH interacts with the amino-terminal domain of XRCC1, and APEH facilitates both single-strand break repair and cell survival following exposure to H2O2. Thus, APEH as a novel proteolytic component of the DNA damage response. PMID: 28866241
2. APEH polymorphism has significant influence on valproic acid pharmacokinetics in Chinese population PMID: 27406852
3. Studies indicate that the cylindromatosis/turban tumor syndrome gene (CYLD) ranked highest, followed by acylaminoacyl-peptidase (APEH), dystroglycan (DAG1), macrophage-stimulating protein (MST1) and ubiquitin-specific peptidase 4 (USP4). PMID: 21931648
亞細胞定位：

Cytoplasm.
蛋白家族：

Peptidase S9C family
數(shù)據(jù)庫鏈接：

HGNC: 586

OMIM: 102645

KEGG: hsa:327

STRING: 9606.ENSP00000296456

UniGene: Hs.517969