Human Activin A,ACV-A ELISA Kit
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中文名稱：人活化素A(ACV-A)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E04486h
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規(guī)格：96T/48T
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價(jià)格：￥3200/￥2500
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：人活化素A(ACV-A)酶聯(lián)免疫試劑盒（CSB-E04486h）為雙抗夾心法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的ACV-A含量。人活化素A（ACV-A）是TGF-β超家族成員，參與生殖、胚胎發(fā)育、紅細(xì)胞分化等過程。其研究機(jī)制涉及調(diào)控細(xì)胞增殖、凋亡和炎癥反應(yīng)，與多種疾病如腫瘤、炎癥性腸病等發(fā)病機(jī)制相關(guān)。試劑盒檢測(cè)范圍為15.6 pg/mL-1000 pg/mL，靈敏度為3.9 pg/mL。適用于基礎(chǔ)科研中探究ACV - A在生殖生物學(xué)、炎癥反應(yīng)、纖維化疾病及腫瘤微環(huán)境中的表達(dá)調(diào)控機(jī)制，尤其適合評(píng)估病理模型或藥物干預(yù)后體液或組織內(nèi)ACV - A的動(dòng)態(tài)變化，為信號(hào)通路研究、生物標(biāo)志物篩選提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：ACV-A
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種屬：Homo sapiens (Human)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：15.6 pg/mL-1000 pg/mL
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靈敏度：3.9 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Signal Transduction
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測(cè)定原理：quantitative
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測(cè)定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Intra-Assay Precision
Inter-Assay Precision
Sample
1
2
3
1
2
3
n
20
20
20
20
20
20
Mean(pg/ml)
125.907
124.505
129.620
124.680
126.082
125.731
SD
0.037
0.035
0.042
0.045
0.048
0.049
CV(%)
3.532
3.366
3.930
4.323
4.576
4.681
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線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of human ACV-A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
Sample
Serum(n=4)
1:1
Average %
84
Range %
80-92
1:2
Average %
95
Range %
90-99
1:4
Average %
97
Range %
92-101
1:8
Average %
105
Range %
100-110
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回收率：
The recovery of human ACV-A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type
Average % Recovery
Range
Serum (n=5)
90
85-94
EDTA plasma (n=4)
93
89-97
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標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml
OD1
OD2
Average
Corrected
1000
3.254
3.125
3.190
3.072
500
2.329
2.414
2.372
2.254
250
1.588
1.626
1.607
1.489
125
1.098
1.040
1.069
0.951
62.5
0.677
0.634
0.656
0.538
31.2
0.395
0.377
0.386
0.268
15.6
0.206
0.198
0.202
0.084
0
0.117
0.118
0.118
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數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- H Antigen expression modulates epidermal Keratinocyte Integrity and differentiation SP Jin, JH Oh, NK Kim, JH Chung,Biological Research,2024
- Effects of Icelandic yogurt consumption and resistance training in healthy untrained older males R Bagheri,British Journal of Nutrition,2021
- Comparison of whole egg v. egg white ingestion during 12 weeks of resistance training on skeletal muscle regulatory markers in resistance-trained men R Bagheri,Br J Nutr,2020
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相關(guān)產(chǎn)品
靶點(diǎn)詳情
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最新研究進(jìn)展：激活素A（Activin A）是一種細(xì)胞因子，能夠在機(jī)體內(nèi)調(diào)節(jié)細(xì)胞增殖、分化、凋亡等生物學(xué)過程。近年來，激活素A的研究逐漸深入，以下是最新的研究進(jìn)展：激活素A在癌癥中的作用機(jī)制得到了深入研究。研究人員發(fā)現(xiàn)，激活素A能夠促進(jìn)癌細(xì)胞的增殖和轉(zhuǎn)移，并且在多種腫瘤中高表達(dá)。此外，激活素A還能夠影響腫瘤免疫逃逸，從而影響腫瘤的發(fā)展和治療效果。激活素A與心血管疾病的關(guān)聯(lián)性研究也得到了廣泛關(guān)注。研究人員發(fā)現(xiàn)，激活素A能夠促進(jìn)血管內(nèi)皮細(xì)胞的增殖和遷移，從而影響血管生成和修復(fù)。同時(shí)，激活素A還能夠促進(jìn)血小板聚集，從而增加血栓形成的風(fēng)險(xiǎn)。激活素A在肝臟疾病中的作用也成為研究熱點(diǎn)。研究人員發(fā)現(xiàn)，激活素A能夠影響肝臟細(xì)胞的增殖和凋亡，并且在肝癌和肝纖維化等疾病中高表達(dá)。激活素A在治療中的應(yīng)用也取得了一些進(jìn)展。例如，研究人員發(fā)現(xiàn)激活素A能夠促進(jìn)骨骼肌再生和修復(fù)，從而在骨骼肌疾病的治療中具有潛在應(yīng)用價(jià)值。
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