Horse insulin (INS) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

馬胰島素(INS)酶聯(lián)免疫試劑盒（CSB-EL011742HO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的INS含量。INS即胰島素基因，其編碼的胰島素是調(diào)節(jié)血糖的關鍵激素。在背景上，胰島素分泌異常與糖尿病等疾病密切相關。研究機制方面，圍繞其基因表達調(diào)控、蛋白合成加工及分泌等展開，旨在揭示疾病發(fā)病機制，為糖尿病等治療提供理論基礎。試劑盒檢測范圍為1.56 nIU/mL-100 nIU/mL，該產(chǎn)品可應用于動物科研領域，例如研究馬的糖代謝調(diào)控機制、胰島素相關疾病模型構建、營養(yǎng)干預或藥物作用下的胰島素分泌變化分析，以及運動生理學中能量代謝的動態(tài)監(jiān)測等方向，為探索胰島素在馬體內(nèi)的生物學功能提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

INS ELISA Kit; Insulin [Cleaved into: Insulin B chain; Insulin A chain] ELISA Kit
縮寫：

INS
Uniprot No.：

P01310
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

1.56 nIU/mL-100 nIU/mL
靈敏度：

0.39 nIU/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	99
	Range %	96-102
1:2000	Average %	87
	Range %	84-91
1:4000	Average %	95
	Range %	90-99
1:8000	Average %	98
	Range %	94-102

回收率：

The recovery of horse INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	104	100-108

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.
亞細胞定位：

Secreted.
蛋白家族：

Insulin family