Horse hypoxia inducible factor 1, alpha subunit (basic helix-loop-helix transcription factor) (HIF1A) ELISA kit

說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

馬缺氧誘導(dǎo)因子1，α亞基(HIF1A)酶聯(lián)免疫試劑盒（CSB-EL010351HO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的HIF1A含量。HIF1A是一個重要靶點。它是低氧誘導(dǎo)因子1的α亞基，在細(xì)胞對低氧環(huán)境應(yīng)答中起關(guān)鍵作用。研究發(fā)現(xiàn)其參與腫瘤生長、轉(zhuǎn)移和血管生成等過程。針對它的研究機(jī)制多聚焦于調(diào)控其表達(dá)及活性，以尋找抗癌等疾病的新療法。試劑盒檢測范圍為31.25 pg/mL-2000 pg/mL，如通過檢測動物模型血清/組織中的HIF1A水平，評估缺氧相關(guān)信號通路活性或藥物對缺氧適應(yīng)性的調(diào)控作用，還能為心血管疾病、腫瘤生物學(xué)等領(lǐng)域的體外實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

HIF1A
Uniprot No.：

F6VG41
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

31.25 pg/mL-2000 pg/mL
靈敏度：

7.81 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Epigenetics and Nuclear Signaling
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse HIF1A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-94
1:2	Average %	102
	Range %	96-107
1:4	Average %	92
	Range %	87-98
1:8	Average %	94
	Range %	89-98

回收率：

The recovery of horse HIF1A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	103	92-107

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.














pg/ml	OD1	OD2	Average	Corrected
2000	1.899	1.957	1.928	1.819
1000	1.417	1.496	1.457	1.348
500	0.926	0.978	0.952	0.843
250	0.583	0.572	0.578	0.469
125	0.345	0.353	0.349	0.240
62.5	0.246	0.252	0.249	0.140
31.25	0.171	0.178	0.175	0.066
0	0.108	0.109	0.109