Horse Gelsolin(GSN) ELISA kit

Horse Gelsolin(GSN) ELISA kit

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中文名稱：

馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EL009965HO
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

馬凝溶膠蛋白(GSN)酶聯(lián)免疫試劑盒（CSB-EL009965HO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的GSN含量。GSN 是一個(gè)靶點(diǎn)。它在細(xì)胞中具有重要作用，與細(xì)胞的運(yùn)動(dòng)、形態(tài)維持等相關(guān)。研究機(jī)制方面，其可能參與調(diào)節(jié)細(xì)胞骨架的動(dòng)態(tài)變化。通過(guò)對(duì)它的深入研究，有望揭示疾病發(fā)生發(fā)展機(jī)制，為開發(fā)新的治療方法和藥物提供方向。試劑盒檢測(cè)范圍為12.5 ng/mL-800 ng/mL，該產(chǎn)品可為馬屬動(dòng)物細(xì)胞遷移機(jī)制、炎癥相關(guān)疾病模型或肌肉組織再生等基礎(chǔ)研究提供關(guān)鍵數(shù)據(jù)支持，適用于實(shí)驗(yàn)室條件下對(duì)生物樣本中GSN蛋白表達(dá)水平的動(dòng)態(tài)監(jiān)測(cè)及比較分析，為探索其分子功能及調(diào)控機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說(shuō)明書。
別名：

GSNGelsolin ELISA kit; Actin-depolymerizing factor ELISA kit; ADF ELISA kit; Brevin ELISA kit
縮寫：

GSN
Uniprot No.：

Q28372
種屬：

Equus caballus (Horse)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

12.5 ng/mL-800 ng/mL
靈敏度：

6.25 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Metabolism
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of horse GSN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	101
	Range %	94-105
1:2000	Average %	89
	Range %	82-94
1:4000	Average %	102
	Range %	98-106
1:8000	Average %	95
	Range %	91-99

回收率：

The recovery of horse GSN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-97
EDTA plasma (n=4)	91	87-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
800	0.234	0.228	0.231
400	0.462	0.453	0.458
200	0.710	0.684	0.697
100	1.028	1.001	1.015
50	1.276	1.245	1.261
25	1.584	1.532	1.558
12.5	1.683	1.623	1.653
0	1.827	1.789	1.808

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Synovial fluid alpha‐2‐macroglobulin, gelsolin and lubricin distinguish between osteoarthritic and healthy equine joints EJ Secor, SJ Womack, MP Ysebaert,Equine Veterinary Journal,2025

功能：

Calcium-regulated, actin-modulating protein that binds to the plus (or barbed) ends of actin monomers or filaments, preventing monomer exchange (end-blocking or capping). It can promote the assembly of monomers into filaments (nucleation) as well as sever filaments already formed. Plays a role in ciliogenesis.
基因功能參考文獻(xiàn)：
1. Ca2+ binding by domain 2 plays a critical role in the activation and stabilization of gelsolin PMID: 19666512
亞細(xì)胞定位：

Cytoplasm, cytoskeleton.
蛋白家族：

Villin/gelsolin family
數(shù)據(jù)庫(kù)鏈接：

KEGG: ecb:100034186

UniGene: Eca.2517

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.