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Guinea pig thyroxine (T4) ELISA kit

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產(chǎn)品詳情

產(chǎn)品描述：

豚鼠甲狀腺素(T4)酶聯(lián)免疫試劑盒（CSB-EQ027512GU）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿樣本中的T4含量。豚鼠甲狀腺素T4是調(diào)節(jié)代謝的重要激素，其靶點(diǎn)背景涉及細(xì)胞角蛋白28抗體等，研究機(jī)制方面，包括其與細(xì)胞骨架、上皮細(xì)胞功能等的關(guān)系，以及其在動(dòng)物模型中的應(yīng)用。試劑盒檢測(cè)范圍為20 ng/mL-320 ng/mL，靈敏度為20 ng/mL。該產(chǎn)品可廣泛應(yīng)用于豚鼠血清或血漿樣本的甲狀腺功能研究，包括內(nèi)分泌系統(tǒng)調(diào)控機(jī)制探索、代謝性疾病模型構(gòu)建、環(huán)境污染物對(duì)甲狀腺干擾效應(yīng)評(píng)估等科研領(lǐng)域本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

T4
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma
檢測(cè)范圍：

20 ng/mL-320 ng/mL
靈敏度：

20 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig T4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	85-100
1:2	Average %	90
	Range %	81-99
1:4	Average %	92
	Range %	85-100
1:8	Average %	90
	Range %	90-100

回收率：

The recovery of Guinea pig T4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-99
EDTA plasma (n=4)	93	85-100

最新研究進(jìn)展：

T4是一種甲狀腺激素，它對(duì)機(jī)體的代謝、生長(zhǎng)和發(fā)育都起著重要作用。近年來，T4的研究主要聚焦于其在人類疾病中的作用。例如，有研究發(fā)現(xiàn)，T4水平降低與代謝綜合征和糖尿病的發(fā)生有關(guān)，同時(shí)T4的治療可能對(duì)改善這些疾病有益。此外，T4還與阿爾茨海默病和其他神經(jīng)系統(tǒng)疾病有關(guān)，研究表明T4的調(diào)節(jié)可能有助于改善這些疾病的癥狀。