Guinea pig interleukin 13,IL-13 ELISA Kit

文獻(xiàn)引用 (3) 說明書下載

產(chǎn)品詳情

產(chǎn)品描述：

豚鼠白介素13(IL-13)酶聯(lián)免疫試劑盒（CSB-E12916Gp）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL13含量。IL13是一種免疫調(diào)節(jié)細(xì)胞因子，參與過敏性炎癥和癌癥的發(fā)病機(jī)制。主要由活化的TH2細(xì)胞分泌，與多種細(xì)胞相互作用，調(diào)節(jié)免疫反應(yīng)。研究IL13有助于了解相關(guān)疾病的發(fā)生和發(fā)展，為疾病治療提供新靶點(diǎn)。試劑盒檢測范圍為7.5 pg/mL-300 pg/mL，適用于豚鼠感染模型、呼吸道炎癥研究、免疫調(diào)節(jié)藥物開發(fā)等科研場景，能夠穩(wěn)定檢測包括肺組織勻漿在內(nèi)的多種生物樣本，為探索IL-13在免疫調(diào)控網(wǎng)絡(luò)中的作用及評估實(shí)驗(yàn)性治療方案的生物學(xué)效應(yīng)提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-13
Uniprot No.：

H0W834
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

7.5 pg/mL-300 pg/mL
靈敏度：

3.75 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of Guinea pig IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	83-92
1:2	Average %	95
	Range %	85-105
1:4	Average %	93
	Range %	86-102
1:8	Average %	97
	Range %	92-110

回收率：

The recovery of Guinea pig IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	91-103
EDTA plasma (n=4)	93	86-99

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.