Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

Guinea pig Tumor necrosis factor α,TNF-α ELISA Kit

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中文名稱：

豚鼠腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒
貨號：

CSB-E06772Gu
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

豚鼠腫瘤壞死因子α(TNF-α)酶聯免疫試劑盒（CSB-E06772Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TNF含量。腫瘤壞死因子（TNF）是一種重要的細胞因子。其背景上，它在免疫調節、炎癥反應等生理過程中有關鍵作用。研究機制方面，TNF可通過與受體結合，激活多條信號通路，進而調控細胞的存活、凋亡及炎癥因子釋放等，在多種疾病發病機制中扮演重要角色。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，該產品適用于基礎科研中炎癥機制研究、藥物干預效果評價以及豚鼠模型的免疫相關疾病實驗，可通過檢測組織勻漿中的 TNF-α 水平分析局部炎癥反應，或利用血液樣本評估系統性免疫激活狀態，其表達水平的變化常用于評估感染性疾病、自身免疫反應或腫瘤研究中的免疫狀態。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

TNF ELISA Kit; TNFA ELISA Kit; TNFSF2 ELISA Kit; Tumor necrosis factor ELISA Kit; Cachectin ELISA Kit; TNF-alpha ELISA Kit; Tumor necrosis factor ligand superfamily member 2 ELISA Kit; TNF-a) [Cleaved into: Tumor necrosis factor ELISA Kit; membrane form ELISA Kit; N-terminal fragment ELISA Kit; NTF); Intracellular domain 1 ELISA Kit; ICD1); Intracellular domain 2 ELISA Kit; ICD2); C-domain 1; C-domain 2; Tumor necrosis factor ELISA Kit; soluble form] ELISA Kit
縮寫：

TNF
Uniprot No.：

P51435
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of guinea pig TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	90-100
1:2	Average %	94
	Range %	89-98
1:4	Average %	92
	Range %	87-96
1:8	Average %	93
	Range %	89-100

回收率：

The recovery of guinea pig TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	88-97
EDTA plasma (n=4)	97	92-103

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.350	2.376	2.363	2.200
100	1.884	1.902	1.893	1.730
50	1.368	1.379	1.374	1.211
25	0.961	0.973	0.967	0.804
12.5	0.664	0.681	0.673	0.510
6.25	0.475	0.486	0.481	0.318
3.12	0.305	0.309	0.307	0.144
0	0.162	0.164	0.163

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Suhuang Antitussive Capsule Ameliorates Corticosteroid Insensitivity in Cough Variant Asthma Guinea Pigs by Inhibiting p38 MAPK Signal Pathway T Lyu,Evidence-based complementary and alternative medicine,2023
Effect of Different Levels of Niacin on Serum Biochemical Parameters, Antioxidant Status, Cytokine Levels, Inflammatory Gene Expression and Colonic Microbial Composition in Weaned Piglets Z Wang,Animals,2022
Effects of resveratrol and its derivative pterostilbene on hepatic injury and immunological stress of weaned piglets challenged with lipopolysaccharide Y Li,Journal of Animal Science,2022
Intrauterine Growth Retardation Affects Intestinal Health of Suckling Piglets via Altering Intestinal Antioxidant Capacity, Glucose Uptake, Tight Junction, and Immune Responses X Tang,Oxidative Medicine and Cellular Longevity,2022
Effects of early protein restriction on the growth performance and gut development of pigs fed diets with or without antibiotic Zhao X,animal,2019
Initial Immune Response in Escherichia coli, Staphylococcus aureus, and Candida albicans Bacteremia Vaios Spyropoulos, et al,Inflammation,2019
Maternal dietary resveratrol alleviates weaning-associated diarrhea and intestinal inflammation in pig offspring by changing intestinal gene expression and microbiota Qingwei Meng, et al,Food & Function,2019
Isoleucine attenuates infection induced by E. coli challenge through modulation of intestinal endogenous antimicrobial peptide expression and inhibition of the increase of plasma endotoxin and IL-6 in weaned pigs Man Ren,Food & Function,2019
Mycoplasma hyopneumoniae Mhp597 is a Cytotoxicity, Inflammation and Immunosuppression Associated Nuclease PengLi,Veterinary Microbiology,2019
Effects of Clostridium butyricum or in combination with Bacillus licheniformis on the growth performance, blood indexes, and intestinal barrier function of weanling piglets Zong X, et al,Livestock Science,2018
Effects of dietary supplementation with essential oils and organic acids on the growth performance, immune system, faecal volatile fatty acids and microflora community in weaned piglets Yang CM, et al,Journal of Animal Science,2018
Oyster crude polysaccharides attenuates lipopolysaccharide-induced cytokines production and PPARγ expression in weanling piglets. Yin G.et al,Springerplus.,2016
New Omega-3 Derivatives Reduce Airway Inflammation and Prevent Rho-Kinase Activation in an Allergic Model of Asthma Caroline Mori et al,Journal of Allergy & Therapy,2012

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平均分:

5.0分 - 1 個評價

樣品類型：組織勻漿液肺組織勻漿上清

樣品信息：其他 SPF級豚鼠

稀釋比：沒有稀釋

產品評價：我通過使用CSB-E06772Gu檢測了PR8病毒感染的小鼠肺組織勻漿上清，操作簡單方便易上手，靈敏度非常高，檢測結果十分精準

By 鄧老師

功能：

Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation. Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance. Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6.; The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells.
亞細胞定位：

Cell membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, membrane form]: Membrane; Single-pass type II membrane protein.; [Tumor necrosis factor, soluble form]: Secreted.; [C-domain 1]: Secreted.; [C-domain 2]: Secreted.
蛋白家族：

Tumor necrosis factor family
數據庫鏈接：

STRING: 10141.ENSCPOP00000002129

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.