Guinea pig Interferon γ,IFN-γ ELISA Kit

產品詳情

文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

豚鼠γ干擾素(IFN-γ)酶聯免疫試劑盒（CSB-E06763Gu）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IFN-γ含量。IFN-γ即γ-干擾素，是具有多種生物學活性的細胞因子。背景上，它在免疫調節等機體生理功能中扮演重要角色。研究機制方面，其可激活巨噬細胞、增強NK細胞活性等，在抗感染、抗腫瘤及免疫相關疾病研究中受廣泛關注。試劑盒檢測范圍為18.75 pg/mL-1200 pg/mL，尤其適用于豚鼠模型的感染性疾病機制探索和疫苗開發等科研領域；適用于評估免疫激活狀態或炎癥反應強度；該產品廣泛應用于基礎醫學、獸醫學研究中宿主免疫應答分析、病原體感染模型建立以及生物制劑效果評價等非診斷性科研場景。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

IFN-gamma，interferon gamma
縮寫：

IFN-γ
種屬：

Guinea Sus scrofa (Pig)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

18.75 pg/mL-1200 pg/mL
靈敏度：

4.7 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of pig IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	81-92
1:2	Average %	97
	Range %	92-103
1:4	Average %	93
	Range %	88-108
1:8	Average %	101
	Range %	96-105

回收率：

The recovery of pig IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-102
EDTA plasma (n=4)	90	86-95

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.