Grouper Vitellogenin,VTG ELISA Kit

產(chǎn)品詳情

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文獻(xiàn)引用 (9) 說(shuō)明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

石斑魚卵黃蛋白原(VTG)酶聯(lián)免疫試劑盒（CSB-E14116Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的VTG含量。試劑盒檢測(cè)范圍為50 ng/mL-2000 ng/mL，適用于水產(chǎn)養(yǎng)殖中魚類繁殖力評(píng)估、環(huán)境污染對(duì)生殖系統(tǒng)影響的機(jī)制研究，以及水生態(tài)毒理學(xué)領(lǐng)域的生物監(jiān)測(cè)實(shí)驗(yàn)。為海洋生物生殖發(fā)育研究及生態(tài)毒理評(píng)估提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
別名：

vtg1 ELISA Kit; Vitellogenin ELISA Kit; VTG) [Cleaved into: Lipovitellin I ELISA Kit; LVI); Phosvitin ELISA Kit; PV); Lipovitellin II ELISA Kit; LVII)] ELISA Kit
縮寫：

VTG
Uniprot No.：

Q5IGP8
種屬：

Grouper
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

50 ng/mL-2000 ng/mL
靈敏度：

50 ng/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of grouper VTG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	84-97
1:2	Average %	97
	Range %	91-102
1:4	Average %	95
	Range %	88-103
1:8	Average %	96
	Range %	91-101

回收率：

The recovery of grouper VTG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-100
EDTA plasma (n=4)	95	91-101