Fish dopamine(DA) ELISA kit

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中文名稱：

魚多巴胺(DA)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-EQ027496FI
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

魚多巴胺(DA)酶聯(lián)免疫試劑盒（CSB-EQ027496FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的DA含量。DA即多巴胺，是腦內(nèi)重要神經(jīng)遞質(zhì)，在運(yùn)動(dòng)控制、認(rèn)知、情感及內(nèi)分泌調(diào)節(jié)等過程中發(fā)揮關(guān)鍵作用。研究機(jī)制方面，圍繞其合成、釋放、與受體結(jié)合及代謝等環(huán)節(jié)展開，探究其在帕金森病、精神分裂癥等疾病中異常變化以尋找治療靶點(diǎn)。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，該產(chǎn)品適用于魚類神經(jīng)生物學(xué)研究、水產(chǎn)養(yǎng)殖健康監(jiān)測、環(huán)境污染物對(duì)水生生物神經(jīng)系統(tǒng)的毒性評(píng)估等科研領(lǐng)域，特別在水體環(huán)境毒理學(xué)實(shí)驗(yàn)中，可通過檢測魚類血液或組織DA水平評(píng)估重金屬、農(nóng)藥等污染物對(duì)中樞神經(jīng)系統(tǒng)的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

DA
種屬：

Fish
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

62.5 pg/mL-1000 pg/mL
靈敏度：

62.5 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Neuroscience
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish DA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	94
	Range %	91-98
1:4	Average %	102
	Range %	98-106
1:8	Average %	88
	Range %	82-94

回收率：

The recovery of fish DA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-98
EDTA plasma (n=4)	103	98-107