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中文名稱：魚腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒
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貨號：CSB-E13254Fh
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規(guī)格：96T/48T
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價格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：魚腫瘤壞死因子α(TNF-α)酶聯(lián)免疫試劑盒（CSB-E13254Fh）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的TNF-α含量。TNF-α，即腫瘤壞死因子-α，是一種重要的細(xì)胞因子，主要由活化的巨噬細(xì)胞產(chǎn)生。它通過與其受體TNFR1結(jié)合，激活多種信號通路，影響細(xì)胞增殖、凋亡和炎癥反應(yīng)。TNF-α在多種疾病如炎癥、自身免疫性疾病中起關(guān)鍵作用，其研究有助于開發(fā)新的治療策略。試劑盒檢測范圍為125 pg/mL-5000 pg/mL，本試劑盒適用于魚類免疫相關(guān)研究，如病原感染模型中的炎癥反應(yīng)監(jiān)測、疫苗免疫效果評估、水產(chǎn)養(yǎng)殖動物健康狀態(tài)分析等科研場景，為水生生物免疫學(xué)機制研究及水產(chǎn)疾病防控提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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縮寫：TNF-α
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Uniprot No.：
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種屬：Fish
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樣本類型：serum, plasma
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檢測范圍：125 pg/mL-5000 pg/mL
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靈敏度：125 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish TNF-α in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 95 Range % 89-100 1:2 Average % 93 Range % 87-99 1:4 Average % 95 Range % 88-103 1:8 Average % 96 Range % 90-102 -
回收率：
The recovery of fish TNF-α spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 95-102 EDTA plasma (n=4) 95 91-101 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 5000 0.154 0.156 0.155 2500 0.351 0.328 0.340 1150 0.562 0.535 0.549 375 0.861 0.864 0.863 125 1.544 1.530 1.537 0 2.387 2.355 2.371 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻
- Mitigation of benzo(a)pyrene-induced hepatotoxicity and oxidative damage in Nile tilapia (Oreochromis niloticus) using N-acetylcysteine-loaded starch nanoparticles in porous alginate beads through the CYP1A/Nrf2/HO-1 pathway M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
- TNF-Α, IL-6, HSP-70, fish growth hormone, and growth performance of sea trout, Salmo trutta (Actinopterygii: Salmoniformes: Salmonidae) after long-term dietary administration of β-glucan and BGN-2 O Revina,/,2023
- Irisin inhibits CCK-8-induced TNF-α production via integrin αVβ5-NF-κB signaling pathways in Nile tilapia (Oreochromis niloticus) W Deng,Fish & shellfish immunology,2023
- Pectin Nanoparticle-Loaded Soft Coral Nephthea sp. Extract as In Situ Gel Enhances Chronic Wound Healing: In Vitro, In Vivo, and In Silico Studies NH Hassan,Pharmaceuticals,2023
- Molecular characterization and immune functions of lipasin in Nile tilapia (Oreochromis niloticus): Involvement in the regulation of tumor necrosis factor-α secretion R Dong,Fish & shellfish immunology,2023
- Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
- Dietary taurine modulates hepatic oxidative status, ER stress and inflammation in juvenile turbot (Scophthalmus maximus L.) fed high carbohydrate diets Yue Zhang,Fish & shellfish immunology,2020
- Inclusion of condensed tannins in Lateolabrax japonicus diets: Effects on growth, nutrient digestibility, antioxidant and immune capacity and copper sulphate stress resistance K Peng,Aquaculture Reports,2020
- Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
- Cytokine Responses in Gills of Capoeta umbla as Biomarkers of Environmental Pollution Danabas D.et al,Water Environ Res.,2016
產(chǎn)品評價
靶點詳情
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功能：Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR.
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亞細(xì)胞定位：Membrane; Single-pass type II membrane protein.
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蛋白家族：Tumor necrosis factor family
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