Fish Interleukin 1β (IL- 1β) ELISA Kit
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中文名稱：魚白介素1β(IL-1β)酶聯(lián)免疫試劑盒
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貨號：CSB-E13259Fh
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規(guī)格：96T/48T
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價格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：魚白介素1β(IL-1β)酶聯(lián)免疫試劑盒（CSB-E13259Fh）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL1B含量。IL1B是重要的炎癥細(xì)胞因子，在多種炎癥性疾病及免疫反應(yīng)中起關(guān)鍵作用。研究機(jī)制上，它參與激活免疫細(xì)胞、調(diào)節(jié)炎癥信號通路。圍繞IL1B的研究聚焦于其在疾病發(fā)生發(fā)展中的作用，為開發(fā)抗炎、免疫調(diào)節(jié)藥物提供靶點(diǎn)。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，本產(chǎn)品廣泛應(yīng)用于水產(chǎn)動物免疫學(xué)研究、魚類病原感染模型構(gòu)建、疫苗效力評估以及水產(chǎn)養(yǎng)殖環(huán)境脅迫對機(jī)體免疫功能影響等科研場景，為深入解析魚類先天免疫機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：il1bInterleukin-1 beta ELISA Kit
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縮寫：IL-1β
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Uniprot No.：
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種屬：Fish
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：3.12 pg/mL-200 pg/mL
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靈敏度：0.78 pg/mL
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反應(yīng)時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領(lǐng)域：Immunology
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測定原理：quantitative
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測定方法：Sandwich
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish IL- 1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 85 Range % 82-89 1:2 Average % 94 Range % 90-99 1:4 Average % 104 Range % 97-108 1:8 Average % 99 Range % 94-106 -
回收率：
The recovery of fish IL- 1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 95 89-102 EDTA plasma (n=4) 89 82-95 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average Corrected 200 2.989 2.778 2.884 2.776 100 2.467 2.432 2.450 2.342 50 1.774 1.866 1.820 1.712 25 0.956 0.932 0.944 0.836 12.5 0.436 0.456 0.446 0.338 6.25 0.258 0.270 0.264 0.156 3.12 0.156 0.155 0.156 0.048 0 0.105 0.111 0.108 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Mitigation of benzo(a)pyrene-induced hepatotoxicity and oxidative damage in Nile tilapia (Oreochromis niloticus) using N-acetylcysteine-loaded starch nanoparticles in porous alginate beads through the CYP1A/Nrf2/HO-1 pathway M Abomosallam, A Ateya, AM Shosha, R Refaat,Aquaculture International,2025
- Effects of mandarin peel powder on growth, biochemical, immune, and intestinal health in Oreochromis niloticus at suboptimal temperatures RM Reda, MIA El-Rahim, DA Elkerdawy,BMC Veterinary Research,2024
- Effects of Low Concentration of Glyphosate‐Based Herbicide on Genotoxic, Oxidative, Inflammatory, and Behavioral Meters in Danio rerio (Teleostei and Cyprinidae) E Bortolon Ribas, G Colombo Dal-Pont,Biochemistry Research International,2024
- Embryonic dexamethasone exposure exacerbates hepatic steatosis and APAP-mediated liver injury in zebrafish C Chang,Ecotoxicology and environmental safety,2024
- Comparison of gut toxicity and microbiome effects in zebrafish exposed to polypropylene microplastics: interesting effects of UV-weathering on microbiome JS Choi,Journal of hazardous materials,2024
- Growth, Nutrient Deposition, Plasma Metabolites, and Innate Immunity are Associated with Feeding Rate in Juvenile Starry Flounder (Platichthys Stellatus) JH Cho,/,/
- Aeromonas veronii detection in Egyptian fish farms with summer tilapia mortality outbreaks and the role of formic acid in limiting its spread RM Reda,Philippine Journal of Science,2023
- Vaccination with bacterial ghosts of Streptococcus iniae and Lactococcus garvieae originated from outbreak of marine fish streptococcosis, induce potential protection against the disease in Nile tilapia, Oreochromis niloticus (Linnaeus, 1758) MMA Hussein,Fish & shellfish immunology,2023
- Transcriptome analysis of immune-related gene expression in Yellow River carp (Cyprinus carpio var.) after challenge with Flavobacterium columnare Y Jia,Microbial pathogenesis,2021
- The effects of four plant extracts on growth, sex reversal, immunological and haemato‐biochemical parameters in Nile tilapia, Oreochmomis niloticus (Linnaeus, 1758) I Ghosal,Aquaculture Research,2020
- Quercetin affects shoaling and anxiety behaviors in zebrafish: Involvement of neuroinflammation and neuron apoptosis J Zhang,Fish & Shellfish Immunology,2020
- Functional and Structural Characterization of Zebrafish ASC Yajuan Li.et al,FEBS J,2018
- Immunomodulation of Lactobacillus pentosus PL11 against Edwardsiella tarda infection in the head kidney cells of the Japanese eel (Anguilla japonica). Birhanu BT.et al,Fish Shellfish Immunol.,2016
- Administration of a Polyphenol-Enriched Feed to Farmed Sea Bass (Dicentrarchus labrax L.) Modulates Intestinal and Spleen Immune Responses. Magrone T.et al,Oxid Med Cell Longev.,2016
產(chǎn)品評價
靶點(diǎn)詳情
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功能：Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production.
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基因功能參考文獻(xiàn)：
- Major changes due to graded levels of olive leaf extract in the diets were observed in blood biochemical parameters. TNFalpha, IL1-beta and IL-8 gene expressions were significanlty up-regulated in OLE 0.1% group compared with others. PMID: 29733961
- The predicted 3D structures of IL-1beta, TNF-alpha, and IL-8 from Oncorhynchus mykiss, Dicentrarchus labrax, and Chionodraco hamatus are reported and the differences between them and their mammalian counterparts investigated. PMID: 21184658
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亞細(xì)胞定位：Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Cytoplasmic vesicle, autophagosome. Secreted.
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蛋白家族：IL-1 family
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組織特異性：All three isoforms are expressed in gill, kidney, liver and spleen. Isoform 1 is also expressed in blood. None of the isoforms are expressed in brain.
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數(shù)據(jù)庫鏈接：
UniGene: Omy.11696
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