Fish Insulin,INS ELISA Kit
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中文名稱：魚胰島素(INS)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-E12123Fh
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：魚胰島素(INS)酶聯(lián)免疫試劑盒（CSB-E12123Fh）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的INS含量。INS即胰島素基因，其表達(dá)產(chǎn)生的胰島素在調(diào)節(jié)血糖穩(wěn)態(tài)中至關(guān)重要。研究機(jī)制方面，圍繞胰島素的分泌、作用信號(hào)通路等展開(kāi)，異常的INS表達(dá)或功能與糖尿病等代謝疾病密切相關(guān)，對(duì)其深入研究有助于揭示疾病發(fā)病機(jī)制及開(kāi)發(fā)新療法。試劑盒檢測(cè)范圍為100 pg/mL-1600 pg/mL，該產(chǎn)品適用于魚類生理學(xué)研究，例如評(píng)估不同生長(zhǎng)階段、營(yíng)養(yǎng)狀態(tài)或環(huán)境脅迫（如水溫、鹽度變化）對(duì)胰島素分泌的影響，也可用于水產(chǎn)養(yǎng)殖中魚類代謝疾病模型的機(jī)制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見(jiàn)產(chǎn)品說(shuō)明書。
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縮寫：
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Uniprot No.：
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種屬：Fish
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：100 pg/mL-1600 pg/mL
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靈敏度：100 pg/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Metabolism
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish INS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 92 Range % 86-100 1:2 Average % 98 Range % 92-103 1:4 Average % 85 Range % 80-89 1:8 Average % 91 Range % 83-99 -
回收率：
The recovery of fish INS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 88-98 EDTA plasma (n=4) 92 90-95 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1600 0.178 0.189 0.184 800 0.322 0.340 0.331 400 0.610 0.634 0.622 200 1.257 1.230 1.244 100 2.335 2.475 2.405 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Dietary functional palatability enhancer improved growth and appetite in largemouth bass (Micropterus salmoides) fed a reduced fish meal diet X Zhi, Y Gu, W Zhao, S Xie, Y Bao, S Morais, D Li,Aquaculture Reports,2025
- Vitamin D regulates glucose metabolism in zebrafish (Danio rerio) by maintaining intestinal homeostasis R Shao,The Journal of nutritional biochemistry,2024
- Vitamin D regulates insulin pathway and glucose metabolism in zebrafish (Danio rerio) R Shao,FASEB journal,2023
- Sequence identification and expression characterization of leptin in the spotted scat, Scatophagus argus WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
- Tip60-mediated Rheb acetylation links palmitic acid with mTORC1 activation and insulin resistance Z Zhao,bioRxiv,2023
- Synergetic effects of concurrent chronic exposure to a mixture of OCPs and high-fat diets on type 2 diabetes and beneficial effects of caloric restriction in female zebrafish HyojinLee,Journal of Hazardous Materials,2022
- The feedback regulation of carbohydrates intake on food intake and appetite in grass carp (Ctenopharyngodon idella) XC Yuan,Fish Physiol Biochem,2021
- Effects of acute hyperglycaemia stress on indices of glucose metabolism and glucose transporter genes expression in cobia (Rachycentron canadum) Ye G, et al,Aquaculture Research,2019
- Comparative analysis of glucose metabolism responses of large yellow croaker Larimichthys crocea fed diet with fish oil and palm oil Wang Q,Fish Physiology and Biochemistry,2019
- High level of dietary soybean oil affects the glucose and lipid metabolism in large yellow croaker Larimichthys crocea through the insulin-mediated PI3K/AKT signaling pathway Gu Z, et al,Comparative Biochemistry And Physiology B-Biochemistry & Molecular Biology,2019
- Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
- Growth response, body composition, plasma metabolites, digestive and antioxidant enzymes activities of Siberian sturgeon (Acipenser baerii, Brandt, 1869) fed different dietary protein and carbohydrate: lipid ratio Babaei S.et al,Aquaculture Research,2016
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