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中文名稱：魚細胞色素P450 1A1(CYP1A1)酶聯免疫試劑盒
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貨號：CSB-EL006395FI
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規格：96T/48T
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價格：￥4200/￥3000
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其他：
產品詳情
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產品描述：魚細胞色素P450 1A1(CYP1A1)酶聯免疫試劑盒（CSB-EL006395FI）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的CYP1A1含量。CYP1A1是一種主要的細胞色素P450酶，廣泛研究其激活致癌化合物的能力。它通過芳香烴受體（AhR）介導的轉錄激活，在代謝環境致癌物和膳食化合物中發揮重要作用。CYP1A1在肺癌等癌癥的發生發展中扮演關鍵角色，其研究機制涉及致癌物激活、基因多態性、以及與AhR等受體的相互作用。試劑盒檢測范圍為75 pg/mL-1200 pg/mL，適用于科研場景中評估魚類暴露于環境毒素后的生理響應、污染物生物有效性研究以及生態毒理學模型的建立。為水生生態系統健康監測、污染物代謝機制研究及水產養殖安全評估提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
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別名：cyp1a1 ELISA kit; cyp1aCytochrome P450 1A1 ELISA kit; EC 1.14.14.1 ELISA kit; CYPIA1 ELISA kit
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縮寫：CYP1A1
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Uniprot No.：
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種屬：Fish
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樣本類型：serum, plasma, tissue homogenates
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檢測范圍：75 pg/mL-1200 pg/mL
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靈敏度：75 pg/mL
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反應時間：1-5h
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樣本體積：50-100ul
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檢測波長：450 nm
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研究領域：Cancer
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測定原理：quantitative
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測定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<15% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<15% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of fish CYP1A1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 91 Range % 86-98 1:2 Average % 100 Range % 92-107 1:4 Average % 85 Range % 80-91 1:8 Average % 88 Range % 83-96 -
回收率：
The recovery of fish CYP1A1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 89 88-95 EDTA plasma (n=4) 94 91-97 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
pg/ml OD1 OD2 Average 1200 0.146 0.147 0.147 600 0.238 0.254 0.246 300 0.524 0.553 0.539 150 1.112 1.135 1.124 75 2.338 2.277 2.308 -
數據處理：
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貨期：3-5 working days
引用文獻
- Liver Structure and Function in Yellowtail Kingfish, Seriola lalandi, in Response to Reduced Fish Meal Diets BH Crowe, JO Harris, TJ McWhorter,Aquaculture, Fish and Fisheries,2024
- Triklorfonun Gammarus pulex'te Baz? Enzimatik Antioksidanlara Etkisinin De?erlendirilmesi ?T Yolcular,Ulusal ?evre Bilimleri Ara?t?rma Dergisi,/
- Dietary Artemisia arborescens Supplementation Effects on Growth, Oxidative Status, and Immunity of Gilthead Seabream (Sparus aurata L.) OP Tzortzatos,Animals,2024
- Dimethoate ve Malathion Pestisitlerinin Dreissena polymorpha (Pallas, 1771) üzerindeki Kombine Etkisinin Sitokrom P450 ve Asetilkolinesteraz Kullan?larak Belirlenmesi NC Yildirim,researchgate,2022
- Toxicological effects of di-(2-ethylhexyl) phthalate in Gammarus pulex: a biochemical and histopathological assessment NC Yildirim,Environmental Science and Pollution Research,2021
- The assessment of basic blue 41 textile dye decolorisation using the Fenton process: response surface methodology optimisation and toxicity evaluation M Tanyol,Chemistry and Ecology,2021
- Evaluation of the efficiency of chlorpyrifos-ethyl remediation by Methylobacterium radiotolerans and Microbacterium arthrosphaerae using response of some biochemical biomarkers GO Erguven,Environmental Science and Pollution Research,2020
- Congo Red Effects on Detoxification and Metabolic Enzymes in Rainbow Trout (Oncorhynchus mykiss Walbaum, 1792) Durali Danabas, et al,Turkish Journal of Agriculture - Food Science and Technology,2019
- Cytochrome P450 and Glutathione-S-Transferase Activity are Altered Following Environmentally Relevant Atrazine Exposures in Crayfish (Faxoniusvirilis) Awali S, et al,Bulletin of Environmental Contamination and Toxicology,2019
- The usability of oxidative stress and detoxification biomarkers in Gammarus pulex for ecological risk assessment of textile dye methyl orange Nuran Cikcikoglu Yildirim, et al,Chemistry And Ecology,2019
- Exposure Through Runoff and Ground Water Contamination Differentially Impact Behavior and Physiology of Crustaceans in Fluvial Systems Alexandra N. Steele.et al,ARCH ENVIRON CON TOX,2018
- Biochemical responses of Gammarus pulex to malachite green solutions decolorized by Coriolus versicolor as a biosorbent under batch adsorption conditions Yildirim NC.et al,Ecotoxicol Environ Saf,2018
產品評價
相關產品
靶點詳情
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功能：Cytochromes P450 are a group of heme-thiolate monooxygenases. They oxidize a variety of structurally unrelated compounds, including steroids, fatty acids, and xenobiotics.
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亞細胞定位：Endoplasmic reticulum membrane; Peripheral membrane protein. Microsome membrane; Peripheral membrane protein.
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蛋白家族：Cytochrome P450 family
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數據庫鏈接：
STRING: 8090.ENSORLP00000018073
UniGene: Ola.6266
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