Fish (Salmon) leptin (LEP) ELISA kit

Fish (Salmon) leptin (LEP) ELISA kit

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文獻引用 (13) 說明書下載

中文名稱：

魚(鮭魚)瘦素(LEP)酶聯免疫試劑盒
貨號：

CSB-EL012870FI
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

產品詳情
產品評價
引用文獻
相關問答

產品描述：

魚(鮭魚)瘦素(LEP)酶聯免疫試劑盒（CSB-EL012870FI）為競爭法ELISA試劑盒，定量檢測血清、血漿樣本中的LEP含量。LEP即瘦素基因編碼蛋白瘦素，其在能量平衡和體重調控中扮演關鍵角色。研究機制主要是，瘦素能作用于下丘腦，通過信號傳導調節食欲、能量消耗等。當下，圍繞LEP在治療肥胖、代謝性疾病等方面的研究不斷開展，以發掘其更多治療潛力。試劑盒檢測范圍為62.5 pg/mL-1000 pg/mL，適用于魚類生理學、營養學及環境毒理學研究，可用于探究餌料成分、溫度變化或污染物暴露對魚類代謝調控的影響，為水產養殖優化和生態保護研究提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

LEP
Uniprot No.：

D6BP01
種屬：

Fish(Salmon)
樣本類型：

serum, plasma
檢測范圍：

62.5 pg/mL-1000 pg/mL
靈敏度：

31.25 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Metabolism
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of fish LEP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	91
	Range %	86-98
1:40	Average %	97
	Range %	91-102
1:80	Average %	84
	Range %	80-87
1:160	Average %	90
	Range %	83-96

回收率：

The recovery of fish LEP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	97	93-102

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
1000	0.082	0.086	0.084
500	0.190	0.200	0.195
250	0.483	0.444	0.464
125	0.945	0.959	0.952
62.5	1.672	1.555	1.614
0	2.591	2.670	2.631

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

Dietary functional palatability enhancer improved growth and appetite in largemouth bass (Micropterus salmoides) fed a reduced fish meal diet X Zhi, Y Gu, W Zhao, S Xie, Y Bao, S Morais, D Li,Aquaculture Reports,2025
Potential role of dietary white poplar (Populus alba L.) in stimulating growth, digestion, and antioxidant/immune status of Nile tilapia (Oreochromis niloticus) RE Ibrahim,/,2024
Sequence identification and expression characterization of leptin in the spotted scat, Scatophagus argus WX Li,Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology,2023
The feedback regulation of carbohydrates intake on food intake and appetite in grass carp (Ctenopharyngodon idella) XC Yuan,Fish Physiol Biochem,2021
Lipid Deposition and Mobilisation in Atlantic Salmon Adipocytes M Bou,international journal of molecular sciences,2020
Comparative analysis of glucose metabolism responses of large yellow croaker Larimichthys crocea fed diet with fish oil and palm oil Wang Q,Fish Physiology and Biochemistry,2019
High level of dietary soybean oil affects the glucose and lipid metabolism in large yellow croaker Larimichthys crocea through the insulin-mediated PI3K/AKT signaling pathway Gu Z, et al,Comparative Biochemistry And Physiology B-Biochemistry & Molecular Biology,2019
Stress, cortisol, and feed intake in rainbow trout (Oncorhynchus mykiss) - Can you stomach the stress? Hoyland, Maren.et al,/,2018
Chronic stress of high dietary carbohydrate level causes inflammation and influences glucose transport through SOCS3 in Japanese flounder Paralichthys olivaceus Kangyu Deng.et al,SCI REP-UK,2018
Physiological responses of Atlantic salmon (Salmo salar L.) fed very low (3%) fishmeal diets supplemented with feeding-modulating crystalline amino acid Katerina Kousoulaki.et al,Aquaculture,2017
Adiponectin Attenuates Lung Fibroblasts Activation and Pulmonary Fibrosis Induced by Paraquat Rong Yao, et al,PLoS One.,2015
The Expression of Leptin, Estrogen Receptors, and Vitellogenin mRNAs in Migrating Female Chum Salmon, Oncorhynchus keta: The Effects of Hypo-osmotic Environmental Changes Young Jae Choi et al, Asian Australas. J. Anim. Sci.,2014
Metabolism, health and fillet nutritional quality in Atlantic salmon (Salmo salar) fed diets containing n-3-rich microalgae Katerina Kousoulaki. et al,Journal of Nutritional Science,2015

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■ 常見問題解答

https://www.cusabio.com/ELISA-Kit/Fish-Salmon-leptin-LEP-ELISA-kit-87315.html
Have you ever tested tissue sample?

Thanks for your inquiry!We only tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test tissue samplet,we suggest do pre-testing first.Here is the processing way on tissue samples.Pls check for your reference:
Tissue Homogenates 100mg tissue was rinsed with 1X PBS, homogenized in 1 ml of 1X PBS and stored overnight at -20°C. After two freeze-thaw cycles were performed to break the cell membranes, the homogenates were centrifuged for 5 minutes at 5000 x g, 2 - 8°C. The supernate was removed and assayed immediately. Alternatively, aliquot and store samples at -20°C or -80°C. Centrifuge the sample again after thawing before the assay. Avoid repeated freeze-thaw cycles.

Is this kit also compatible to test Leptin levels in Zebrafish?

Thanks for your inquiry!We suggest you do a pretest if you want to test zebrafish.

Per customer request, can you please provide the following information about this kit:
a. Capture antibody or capture antigen – immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
b. Detection antibody (if applicable) - immunogen type, sequence (including species source and accession no. if available), expression host, clonality and host species.
c. Standard – if recombinant protein, please specify sequence (including species source and accession no. if available) and expression host.
d. Please send the manual.
e. Please provide your reference data for fish plasma samples.

Thanks for your inquiry.
CSB-EL012870FI
This is a competitive kit. There is only one antibody in the kit.
The plate is coated with mouse monoclonal antibody. ( Immunogen is CHO-derived recombinant protein.）
Standard is CHO-derived recombinant protein.
Pls find attached manual.
We tested salmon serum and plasma and some common freshwater fish before and the test value is about
125pg/ml under 1:100 dilution. The final concentration is about 12.5ng/ml.
Pls let me know if you have any further questions. Thank you.

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.