Duck Interleukin 2,IL-2 ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

鴨白介素2(IL-2)酶聯(lián)免疫試劑盒（CSB-E08899Du）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-2含量。IL-2是重要的免疫細(xì)胞因子，其背景在于在免疫調(diào)節(jié)中扮演關(guān)鍵角色。研究機(jī)制上，IL-2能刺激T細(xì)胞和NK細(xì)胞的生長、增殖與活化，增強(qiáng)機(jī)體免疫功能，在腫瘤免疫治療、自身免疫病等研究領(lǐng)域備受關(guān)注，為相關(guān)疾病治療帶來新希望。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，操作流程標(biāo)準(zhǔn)化，支持科研人員高效開展鴨免疫相關(guān)研究，可用于評估病原感染后免疫細(xì)胞活性、炎癥反應(yīng)動態(tài)監(jiān)測或免疫調(diào)節(jié)藥物作用效果分析等實驗場景本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-2
Uniprot No.：

Q804B7
種屬：

Duck
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of duck IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-105
1:2	Average %	92
	Range %	87-100
1:4	Average %	95
	Range %	86-104
1:8	Average %	90
	Range %	81-98

回收率：

The recovery of duck IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	85-105
EDTA plasma (n=4)	94	89-101

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.