Dog von Willebrand Factor,vWF ELISA Kit

Dog von Willebrand Factor,vWF ELISA Kit

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中文名稱：

犬血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯免疫試劑盒
貨號：

CSB-E12759c
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬血管性血友病因子/瑞斯托霉素輔因子(VWF)酶聯免疫試劑盒（CSB-E12759c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的VWF含量。VWF即血管性血友病因子，其在止血過程中發揮關鍵作用。當血管受損時，VWF能介導血小板黏附于受損血管壁。研究機制上多圍繞其結構與功能關系，以及與其他凝血因子相互作用，目前它也是出血性和血栓性疾病研究的重要靶點。試劑盒檢測范圍為0.625 ng/mL-40 ng/mL，該產品適用于科研場景中犬類VWF蛋白表達水平的定量分析，可用于探究凝血相關疾病的分子機制、評估實驗動物模型的血栓或出血性病理狀態，以及篩選影響凝血功能的藥物或化合物研究，為獸醫基礎研究與生物醫學實驗提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

VWF ELISA Kit; F8VWF ELISA Kit; von Willebrand factor ELISA Kit; vWF ELISA Kit
縮寫：

VWF
Uniprot No.：

Q28295
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.625 ng/mL-40 ng/mL
靈敏度：

0.32 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cardiovascular
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog vWF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	101
	Range %	95-107
1:200	Average %	94
	Range %	90-100
1:400	Average %	98
	Range %	94-102
1:800	Average %	88
	Range %	84-93

回收率：

The recovery of dog vWF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-99
EDTA plasma (n=4)	97	90-101

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
40	0.093	0.101	0.097
20	0.209	0.216	0.213
10	0.422	0.441	0.432
5	0.739	0.715	0.727
2.5	1.168	1.124	1.146
1.25	1.602	1.709	1.656
0.625	1.779	1.879	1.829
0	2.170	2.297	2.234

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Important in the maintenance of hemostasis, it promotes adhesion of platelets to the sites of vascular injury by forming a molecular bridge between sub-endothelial collagen matrix and platelet-surface receptor complex, glycoprotein Ibalpha/IX/V. Also acts as a chaperone for coagulation factor VIII, delivering it to the site of injury, stabilizing its heterodimeric structure and protecting it from premature clearance from plasma.
基因功能參考文獻：
1. only the c.1657G variant was found in the homozygous state exclusively in Von Willebrand disease affected dogs, and this variant is the strongest candidate to be the cause of VWD type 2 in the German Wirehaired Pointer and German Shorthaired Pointer breeds PMID: 28696025
2. the CD-31 and vonWillebrand factor (vWF) expressions and the cell morphological alterations ofarachnoid villus endothelium in different stages of animal hydrocephalus PMID: 17593818
3. Expression of mutant VWF proteins confirmed that VWF multimerization is not necessary for Weibel-Palade body creation PMID: 15331450
相關疾病：

Defects in VWF are the cause of von Willebrand disease (VWD) in the Scottish Terrier. VWD is characterized by frequent bleeding. Type I VWD is associated with a deficiency of VWF; type II by normal to decreased plasma level of VWF; type III by a virtual absence of VWF.
亞細胞定位：

Secreted. Secreted, extracellular space, extracellular matrix. Note=Localized to storage granules.
組織特異性：

Plasma.
數據庫鏈接：

KEGG: cfa:399544

STRING: 9615.ENSCAFP00000022453

UniGene: Cfa.111

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.