Dog insulin-like growth factor binding protein 3 (IGFBP3)ELISA kit

產(chǎn)品詳情

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說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬胰島素樣生長因子結(jié)合蛋白3(IGFBP3)酶聯(lián)免疫試劑盒（CSB-EL011097DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IGFBP3含量。IGFBP3是一種重要靶點。它參與胰島素樣生長因子（IGF）信號通路的調(diào)控，在細(xì)胞的生長、增殖、凋亡等過程中發(fā)揮作用。相關(guān)研究機制主要聚焦于其與IGF的結(jié)合，以及對下游信號傳導(dǎo)途徑的影響，為腫瘤等疾病的治療提供新方向。試劑盒檢測范圍為3.12 ng/mL-200 ng/mL，本試劑盒適用于科研場景中犬類生長發(fā)育研究、代謝紊亂模型建立、內(nèi)分泌調(diào)控機制探索等領(lǐng)域，例如通過檢測血清IGFBP3水平評估生長激素軸功能狀態(tài)，或分析營養(yǎng)干預(yù)對組織IGF信號通路的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IGFBP3
Uniprot No.：

F1PQ91
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 ng/mL-200 ng/mL
靈敏度：

0.78 ng/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog IGFBP3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	94
	Range %	91-99
1:40	Average %	96
	Range %	90-100
1:80	Average %	97
	Range %	95-100
1:160	Average %	95
	Range %	89-103

回收率：

The recovery of dog IGFBP3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-98
EDTA plasma (n=4)	96	90-102

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.