Dog gastrin (GAST) ELISA kit

產(chǎn)品詳情

說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產(chǎn)品描述：

犬胃泌素(GAST)酶聯(lián)免疫試劑盒（CSB-EL009273DO）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的GAST含量。GAST是胃泌素基因，胃泌素作為胃腸激素，可調(diào)節(jié)胃酸分泌、促進(jìn)胃腸道黏膜生長等。其研究機(jī)制主要圍繞在消化系統(tǒng)疾病如胃潰瘍、胃癌等方面，探究其在疾病發(fā)生發(fā)展中的作用，以及通過調(diào)控GAST來開發(fā)相關(guān)治療方法。試劑盒檢測范圍為6.25 pg/mL-400 pg/mL，適用于科研領(lǐng)域中對犬科動物胃腸疾病模型構(gòu)建、營養(yǎng)代謝研究、藥物干預(yù)效果評估等實(shí)驗(yàn)需求本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

GAST ELISA Kit; GASGastrin [Cleaved into: Big gastrin ELISA Kit; Gastrin-34 ELISA Kit; G34); Gastrin] ELISA Kit
縮寫：

GAST
Uniprot No.：

P01353
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

6.25 pg/mL-400 pg/mL
靈敏度：

1.56 pg/mL
反應(yīng)時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Signal Transduction
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog GAST in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	92-102
1:4	Average %	85
	Range %	81-88
1:8	Average %	89
	Range %	83-96

回收率：

The recovery of dog GAST spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-96
EDTA plasma (n=4)	89	85-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Gastrin stimulates the stomach mucosa to produce and secrete hydrochloric acid and the pancreas to secrete its digestive enzymes. It also stimulates smooth muscle contraction and increases blood circulation and water secretion in the stomach and intestine.
亞細(xì)胞定位：

Secreted.
蛋白家族：

Gastrin/cholecystokinin family
數(shù)據(jù)庫鏈接：

KEGG: cfa:100685087

STRING: 9615.ENSCAFP00000023420