Dog Matrix metalloproteinase 3,MMP-3 ELISA kit

產品詳情

文獻引用 (3) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

犬基質金屬蛋白酶3(MMP-3)酶聯(lián)免疫試劑盒（CSB-E13621c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織培養(yǎng)上清液、尿液、組織勻漿樣本中的MMP3含量。MMP3 即基質金屬蛋白酶 3，它在組織重塑、炎癥等生理病理過程中扮演重要角色。研究發(fā)現(xiàn)其異常表達與多種疾病如關節(jié)炎、腫瘤發(fā)展相關。機制上，它可降解細胞外基質成分，影響細胞微環(huán)境，促進細胞遷移和腫瘤轉移，是極具潛力的治療靶點。試劑盒檢測范圍為1.56 ng/mL-100 ng/mL，適用于科研場景中犬類生物標志物研究，例如探索炎癥相關疾病的分子機制、評估藥物對組織修復的影響或建立特定病理模型。本品僅用于科研，不用于臨床診斷，產品具體參數(shù)及操作步驟詳見產品說明書。
別名：

MMP3Stromelysin-1 ELISA Kit; SL-1 ELISA Kit; EC 3.4.24.17 ELISA Kit; Matrix metalloproteinase-3 ELISA Kit; MMP-3 ELISA Kit
縮寫：

MMP3
Uniprot No.：

Q6Y4Q5
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, cell culture supernates, urine, tissue homogenates
檢測范圍：

1.56 ng/mL-100 ng/mL
靈敏度：

0.39 ng/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog MMP-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	89
	Range %	85-96
1:4	Average %	93
	Range %	89-97
1:8	Average %	99
	Range %	92-105

回收率：

The recovery of dog MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	87-96

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.
基因功能參考文獻：
1. Findings suggested that increased TGF-beta3 and MMP-3 contribute to the pathogenesis of valvular degeneration associated with chronic mitral valve disease. PMID: 21281193
2. Both mRNA transcript levels and the activities of MMP-3 and MMP-7 are elevated in X-linked Alport syndrome (XLAS) kidneys from dogs. PMID: 15782307
亞細胞定位：

Secreted, extracellular space, extracellular matrix.
蛋白家族：

Peptidase M10A family
數(shù)據(jù)庫鏈接：

KEGG: cfa:403445

STRING: 9615.ENSCAFP00000022195

UniGene: Cfa.3447