Dog Endothelin 1,ET-1 ELISA Kit

Dog Endothelin 1,ET-1 ELISA Kit

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中文名稱：

犬內皮素1(ET-1)酶聯免疫試劑盒
貨號：

CSB-E07002c
規格：

96T/48T
價格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產品描述：

犬內皮素1(ET-1)酶聯免疫試劑盒（CSB-E07002c）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的EDN1含量。EDN1即內皮素-1，是一種具有強烈而持久血管收縮作用的活性肽。在生理上，它參與維持心血管穩態。研究機制方面，聚焦其與心血管、呼吸系統疾病等關聯，探討其信號通路及作用靶點，期望開發針對性藥物干預治療。試劑盒檢測范圍為3.12 pg/mL-200 pg/mL，適用于科研領域對犬血清、血漿及組織勻漿樣本中 ET - 1 含量的精準分析；適用于實驗室對犬模型在基礎研究、藥物作用機制探索或疾病模型構建中的 ET - 1 動態監測本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
別名：

EDN1Endothelin-1 ELISA kit; ET-1 ELISA kit; Preproendothelin-1 ELISA kit; PPET1) [Cleaved into: Big endothelin-1] ELISA kit
縮寫：

EDN1
Uniprot No.：

P13206
種屬：

Canis lupus familiaris (Dog) (Canis familiaris)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

3.12 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Cancer
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of dog ET-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	85-96
1:2	Average %	94
	Range %	90-99
1:4	Average %	89
	Range %	81-96
1:8	Average %	100
	Range %	91-108

回收率：

The recovery of dog ET-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	87	82-96
EDTA plasma (n=4)	97	82-106

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
200	2.954	2.989	2.972	2.861
100	2.388	2.398	2.393	2.282
50	1.758	1.789	1.774	1.663
25	1.035	1.069	1.052	0.941
12.5	0.502	0.543	0.523	0.412
6.25	0.271	0.268	0.270	0.159
3.12	0.184	0.169	0.177	0.066
0	0.112	0.110	0.111

數據處理：

ELISA操作視頻及標曲繪制軟件下載
貨期：

3-5 working days

功能：

Endothelins are endothelium-derived vasoconstrictor peptides. Probable ligand for G-protein coupled receptors EDNRA and EDNRB which activates PTK2B, BCAR1, BCAR3 and, GTPases RAP1 and RHOA cascade in glomerular mesangial cells.
基因功能參考文獻：
1. These findings suggest that elevated serum big ET-1 could be used as a diagnostic marker for canine hemangiosarcoma . PMID: 25920760
2. This study showed that canine osteosarcoma cells express endothelin-1 and functional endothelin A receptors; increases in bone alkaline phosphatase activity are associated with osteoblastic OS lesions. PMID: 26426813
3. There was a progressive increase in ET-1 and ETAR expression from benign tumour to grade 1 and grade 2 malignant mammary tumours with a decrease of expression in grade 3 carcinomas. Co-localization of ET-1 and ETAR was observed in benign mammary tumours. PMID: 25816929
4. These findings point to the potential of serum big ET-1 as a clinical marker for cardiopulmonary and neoplastic diseases in dogs. PMID: 24447631
5. Renal denervation substantially decreased BP and also significantly decreased the plasma levels of renin-angiotensin system and ET-1. PMID: 24496516
6. These results showed that endothelin-1 and endothelin receptor A are overexpressed in canine ovarian tumours, suggesting a potential role of these two molecules in canine ovarian carcinogenesis. PMID: 20210880
7. ET-1 production in macrophage cell line DH82 was increased in response to stimulation with intact and viable gram-positive and gram-negative bacteria. PMID: 20207425
8. The results showed that there was an interaction between ET-1 and NO in a VILI (ventilator-induced lung injury )model and changing the balance of ET-1 and NO levels might contribute to the pathophysiologic process of VILI. PMID: 20497708
9. Report endothelin levels in experimental diabetes combined with cardiac hypertrophy. PMID: 15838277
10. Report changes in endothelin-1 gene expression during myocardial ischemia and reperfusion. PMID: 15838278
11. Intrapericardial adenine nucleosides induce elevation of endothelin-1 concentration in the pericardial space of the dog heart. PMID: 15838286
12. The results confirm that intrapericardial catecholamines exert significant hemodynamic effects and modulate ET-1 and adenosine release from the heart. PMID: 15838288
13. Report changes in endothelin-1/big endothelin-1 levels and action potential duration during myocardial ischemia-reperfusion in dogs with and without ventricular fibrillation. PMID: 15838324
14. although ET(A) receptor-signaling is sensitized by induction of the metabolic syndrome, endothelin-mediated coronary vasoconstriction does not significantly contribute to coronary dysfunction at this early stage of prediabetes PMID: 16627363
15. These results may potentially explain resting perfusion abnormalities or heterogeneity by clinical PET that may persist or only partially improve after adenosine stress perfusion imaging in the absence of myocardial scar and flow-limiting stenosis. PMID: 16951040
16. Alterations in blood levels of endothelin-1, atrial natriuretic peptide, aldosterone, and norepinephrine in dogs with and without dilated cardiomyopathy are reported. PMID: 17338155
17. Early pulmonary hypertension in heart failure is characterized by altered vasoreactivity and increased ET-1 and VEGF2 signalling. PMID: 17993509
18. peripheral NO and ET-1 release modulates the cardiac contractility through myocardial ET-A receptor activation and mast cell degranulation. PMID: 18788466
19. The use of Plasma atrial natriuretic peptide (proANP 31-67), B-type natriuretic peptide (Nt-proBNP) and endothelin-1 (ET-1) concentrations as disease markers in dogs with valvular heart disease is reported. PMID: 19585255
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亞細胞定位：

Secreted.
蛋白家族：

Endothelin/sarafotoxin family
數據庫鏈接：

KEGG: cfa:403424

STRING: 9615.ENSCAFP00000014389

UniGene: Cfa.125

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.