Dog Apolipoprotein A-I(APOA1) ELISA kit
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中文名稱：犬載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒
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貨號(hào)：CSB-EL001913DO
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規(guī)格：96T/48T
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價(jià)格：￥4200/￥3000
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其他：
產(chǎn)品詳情
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產(chǎn)品描述：犬載脂蛋白A1(Apo-A1)酶聯(lián)免疫試劑盒（CSB-EL001913DO）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的APOA1含量。APOA1是一種位于細(xì)胞膜外的分泌蛋白，是高密度脂蛋白（HDL）的主要結(jié)構(gòu)載脂蛋白，在逆向膽固醇轉(zhuǎn)運(yùn)途徑中起關(guān)鍵作用。其研究機(jī)制涉及膽固醇代謝、抗炎、抗動(dòng)脈粥樣硬化等功能，是心血管疾病預(yù)防和治療中的潛在靶點(diǎn)。試劑盒檢測(cè)范圍為28.12 ng/mL-1800 ng/mL，該產(chǎn)品適用于科研領(lǐng)域中對(duì)犬類脂代謝機(jī)制、心血管疾病模型構(gòu)建、營(yíng)養(yǎng)干預(yù)效果評(píng)估等研究，尤其在探究HDL功能與疾病相關(guān)性、藥物或膳食成分對(duì)脂代謝影響的實(shí)驗(yàn)中具有廣泛應(yīng)用。組織勻漿兼容性設(shè)計(jì)還可支持特定器官或病理組織中Apo - A1的分布研究，為深入探索犬類脂質(zhì)代謝的分子機(jī)制提供可靠工具。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
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別名：APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
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縮寫：
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Uniprot No.：
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種屬：Canis lupus familiaris (Dog) (Canis familiaris)
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樣本類型：serum, plasma, tissue homogenates
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檢測(cè)范圍：28.12 ng/mL-1800 ng/mL
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靈敏度：7 ng/mL
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反應(yīng)時(shí)間：1-5h
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樣本體積：50-100ul
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檢測(cè)波長(zhǎng)：450 nm
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研究領(lǐng)域：Cardiovascular
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測(cè)定原理：quantitative
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測(cè)定方法：Competitive
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精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of dog APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1000 Average % 93 Range % 89-103 1:2000 Average % 89 Range % 85-95 1:4000 Average % 95 Range % 84-103 1:8000 Average % 93 Range % 83-98 -
回收率：
The recovery of dog APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 93 92-95 EDTA plasma (n=4) 95 91-99 -
標(biāo)準(zhǔn)曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average 1800 0.142 0.145 0.144 900 0.215 0.229 0.222 450 0.382 0.367 0.375 225 0.551 0.558 0.555 112.5 0.973 0.990 0.982 56.25 1.697 1.623 1.660 28.12 2.287 2.328 2.308 0 2.654 2.761 2.708 -
數(shù)據(jù)處理：
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貨期：3-5 working days
引用文獻(xiàn)
- Oral nicotinic acid administration effect on lipids, thyroid hormones, and oxidative stress in intact adult dogs N Derakhshandeh, S Nazifi, A Mogheiseh,BMC Veterinary Research,2025
產(chǎn)品評(píng)價(jià)
相關(guān)產(chǎn)品
靶點(diǎn)詳情
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功能：Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
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亞細(xì)胞定位：Secreted.
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蛋白家族：Apolipoprotein A1/A4/E family
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組織特異性：Major protein of plasma HDL, also found in chylomicrons. Synthesized in the liver and small intestine.
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數(shù)據(jù)庫鏈接：
STRING: 9615.ENSCAFP00000019630
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