-
中文名稱：雞轉化生長因子β1(TGF-β1)酶聯免疫試劑盒
-
貨號：CSB-E09875Ch
-
規格：96T/48T
-
價格：￥4200/￥3000
-
其他：
產品詳情
-
產品描述：雞轉化生長因子β1(TGF-β1)酶聯免疫試劑盒（CSB-E09875Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的TGFB1含量。TGFB1是重要的靶點，它是一種多功能細胞因子。在細胞生長、分化、凋亡等過程中發揮關鍵作用。其異常表達與多種疾病如腫瘤、纖維化等密切相關。研究機制主要圍繞其信號通路展開，旨在調控其活性來治療相關疾病。試劑盒檢測范圍為0.312 ng/mL-20 ng/mL，適用于家禽免疫學基礎研究、病理模型建立及生物標志物分析，特別在探究禽類傳染性疾病、疫苗效價評估或應激反應機制等科研場景中，能夠為實驗提供穩定可靠的定量數據支持。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
-
別名：TGFB1 ELISA Kit; Transforming growth factor beta-1 proprotein [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-1 ELISA Kit; TGF-beta-1)] ELISA Kit
-
縮寫：
-
Uniprot No.：
-
種屬：Gallus gallus (Chicken)
-
樣本類型：serum, plasma, tissue homogenates
-
檢測范圍：0.312 ng/mL-20 ng/mL
-
靈敏度：0.078 ng/mL
-
反應時間：1-5h
-
樣本體積：50-100ul
-
檢測波長：450 nm
-
研究領域：Signal Transduction
-
測定原理：quantitative
-
測定方法：Sandwich
-
精密度：
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
線性度：
To assess the linearity of the assay, samples were spiked with high concentrations of chicken TGF-β1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:1 Average % 93 Range % 84-97 1:2 Average % 95 Range % 84-98 1:4 Average % 96 Range % 89-103 1:8 Average % 95 Range % 85-100 -
回收率：
The recovery of chicken TGF-β1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 90 84-97 EDTA plasma (n=4) 97 92-102 -
標準曲線：
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. 
ng/ml OD1 OD2 Average Corrected 20 2.011 2.105 2.058 1.965 10 1.790 1.828 1.809 1.716 5 1.466 1.527 1.497 1.404 2.5 1.149 1.163 1.156 1.063 1.25 0.795 0.854 0.825 0.732 0.625 0.433 0.425 0.429 0.336 0.312 0.264 0.255 0.260 0.167 0 0.095 0.091 0.093 -
數據處理：
-
貨期：3-5 working days
引用文獻
- Molecular characterization and immune protection of an AN1-like zinc finger protein of Eimeria tenella Huanzhi Zhao, et al,Parasitology Research,2019
- The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
- Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,Parasites & Vectors,2018
- The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
- Effect of Antibiotic Growth Promoters on Anti-oxidative and Anti-inflammatory Activities in Broiler Chickens Kabploy K.et al,The Thai Journal of Veterinary Medicine,2016
- The molecular characterization and immune protection of microneme 2 of Eimeria acervulina ZC Zhang. et al,Veterinary Parasitology,2015
- Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
- Eimeria maxima microneme protein 2 delivered as DNA vaccine and recombinant protein induces immunity against experimental homogenous challenge Huang J. et al,Parasitol Int,2015
- Effect of garlic component s-allyl cysteine sulfoxide onglycatedhumanserum albumininduced activationof endothelial cells: aninvitro study B.K. NIRALA. et al,European Review for Medical and Pharmacological Sciences,2015
產品評價
相關產品
靶點詳情
-
功能：Transforming growth factor beta-1 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains, which constitute the regulatory and active subunit of TGF-beta-1, respectively.; Required to maintain the Transforming growth factor beta-1 (TGF-beta-1) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-1 and regulates its activation via interaction with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS, that control activation of TGF-beta-1. Interaction with integrins (ITGAV:ITGB6 or ITGAV:ITGB8) results in distortion of the Latency-associated peptide chain and subsequent release of the active TGF-beta-1.; Transforming growth factor beta-1: Multifunctional protein that regulates the growth and differentiation of various cell types and is involved in various processes, such as normal development, immune function, microglia function and responses to neurodegeneration. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-1 (TGF-beta-1) chains remain non-covalently linked rendering TGF-beta-1 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1, LRRC32/GARP and LRRC33/NRROS that control activation of TGF-beta-1 and maintain it in a latent state during storage in extracellular milieus. TGF-beta-1 is released from LAP by integrins (ITGAV:ITGB6 or ITGAV:ITGB8): integrin-binding to LAP stabilizes an alternative conformation of the LAP bowtie tail and results in distortion of the LAP chain and subsequent release of the active TGF-beta-1. Once activated following release of LAP, TGF-beta-1 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal. While expressed by many cells types, TGF-beta-1 only has a very localized range of action within cell environment thanks to fine regulation of its activation by Latency-associated peptide chain (LAP) and 'milieu molecules'. Plays an important role in bone remodeling: acts as a potent stimulator of osteoblastic bone formation. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. Can induce epithelial-to-mesenchymal transition (EMT) and cell migration in various cell types.
-
亞細胞定位：[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-1]: Secreted.
-
蛋白家族：TGF-beta family
-
數據庫鏈接：
UniGene: Gga.929
Most popular with customers
-
Human Transforming Growth factor β1,TGF-β1 ELISA kit
Detect Range: 23.5 pg/ml-1500 pg/ml
Sensitivity: 5.8 pg/ml
-
-
-
Mouse Tumor necrosis factor α,TNF-α ELISA Kit
Detect Range: 7.8 pg/ml-500 pg/ml
Sensitivity: 1.95 pg/ml
-
-
-
-
