Chicken Parathyroid hormone,PTH ELISA Kit

Chicken Parathyroid hormone,PTH ELISA Kit

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文獻(xiàn)引用 (7) 說明書下載

中文名稱：

雞甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E11880Ch
規(guī)格：

96T/48T
價(jià)格：

￥3200/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

雞甲狀旁腺激素(PTH)酶聯(lián)免疫試劑盒（CSB-E11880Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的PTH含量。PTH即甲狀旁腺激素，它主要由甲狀旁腺主細(xì)胞合成和分泌，在調(diào)節(jié)鈣磷代謝、維持血鈣平衡上意義重大。研究發(fā)現(xiàn)PTH能通過與受體結(jié)合激活信號(hào)通路，促進(jìn)骨代謝及腎臟對(duì)鈣的重吸收等，其相關(guān)機(jī)制研究對(duì)骨病等治療有重要潛在價(jià)值。試劑盒檢測(cè)范圍為6.25 pg/mL-100 pg/mL，該產(chǎn)品廣泛應(yīng)用于禽類鈣代謝機(jī)制研究、飼料營(yíng)養(yǎng)評(píng)估、疾病模型構(gòu)建等科研場(chǎng)景，尤其適用于探究環(huán)境因素、遺傳變異或藥物干預(yù)對(duì)禽類甲狀旁腺功能的影響。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

PTH ELISA Kit; Parathyroid hormone ELISA Kit; PTH ELISA Kit
縮寫：

PTH
Uniprot No.：

P15743
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

6.25 pg/mL-100 pg/mL
靈敏度：

6.25 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Signal Transduction
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	86-95
1:2	Average %	96
	Range %	92-103
1:4	Average %	85
	Range %	81-86
1:8	Average %	90
	Range %	83-98

回收率：

The recovery of chicken PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	93	87-98
EDTA plasma (n=4)	94	90-98

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
100	0.184	0.195	0.190
50	0.325	0.350	0.338
25	0.531	0.504	0.518
12.5	1.006	1.026	1.016
6.25	2.117	2.220	2.169

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Effects of a transient lack of dietary mineral phosphorus on renal gene expression and plasma metabolites in two high-yielding laying hen strains H Qasir, H Reyer, M Oster, S Ponsuksili, N Trakooljul,BMC genomics,2025
Dietary Clostridium butyricum and 25-Hydroxyvitamin D3 modulate bone metabolism of broilers through the gut–brain axis G Cao,Poultry science,2024
Circadian regulation of calcium and phosphorus homeostasis during the oviposition cycle in laying hens M Sinclair,Poultry science,2024
Broiler physiological response to low phosphorus diets at different stages of production AO Omotoso,Poultry science,2022
Dietary encapsulated Bacillus subtilis and essential oil supplementation improves reproductive performance and hormone concentrations of broiler breeders during the late laying period Y.B. Chen,Livestock Science,2021
Effects of dietary calcium and available phosphorus levels and phytase supplementation on performance, bone mineral density, serum biochemical bone markers in aged white egg-laying hens. A Bello,Poultry Science,2020
Effects of dietary phosphorus and calcium levels and phytase supplementation on bone metabolism of egg-laying hens Abiodun Bello, et al,/,2018

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功能：

PTH elevates calcium level by dissolving the salts in bone and preventing their renal excretion.
基因功能參考文獻(xiàn)：
1. A single nucleotide polymorphism in parathyroid hormone affected the eggshell percentage and breaking strength, and it may be associated with the variation of serum calcium and PTH level. PMID: 20852100
亞細(xì)胞定位：

Secreted.
蛋白家族：

Parathyroid hormone family
數(shù)據(jù)庫(kù)鏈接：

KEGG: gga:396436

STRING: 9031.ENSGALP00000008579

UniGene: Gga.78

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.