Chicken Interleukin 4,IL-4 ELISA Kit

Chicken Interleukin 4,IL-4 ELISA Kit

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中文名稱：

雞白介素4(IL-4)酶聯(lián)免疫試劑盒
貨號：

CSB-E06756Ch
規(guī)格：

96T/48T
價(jià)格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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雞白介素4(IL-4)酶聯(lián)免疫試劑盒（CSB-E06756Ch）為雙抗夾心法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL-4含量。IL-4是重要的免疫調(diào)節(jié)細(xì)胞因子，在多種免疫和炎癥反應(yīng)中起關(guān)鍵作用。研究發(fā)現(xiàn)它可促進(jìn)B細(xì)胞增殖分化，調(diào)節(jié)Th2細(xì)胞免疫，與哮喘、特應(yīng)性皮炎等疾病的發(fā)生發(fā)展密切相關(guān)。目前圍繞它阻斷信號傳導(dǎo)等機(jī)制的研究，有望帶來新治療策略。試劑盒檢測范圍為12.5 pg/mL-800 pg/mL，適用于科研領(lǐng)域?qū)﹄u免疫系統(tǒng)功能的研究，如傳染病模型中的細(xì)胞因子動態(tài)分析、疫苗免疫效果評估或免疫相關(guān)疾病的分子機(jī)制探索。本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
縮寫：

IL-4
Uniprot No.：

Q5W4U1
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

12.5 pg/mL-800 pg/mL
靈敏度：

3.12 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領(lǐng)域：

Immunology
測定原理：

quantitative
測定方法：

Sandwich

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	80-95
1:2	Average %	94
	Range %	88-101
1:4	Average %	93
	Range %	86-106
1:8	Average %	95
	Range %	82-100

回收率：

The recovery of chicken IL-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-97
EDTA plasma (n=4)	92	85-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	1.726	1.825	1.776	1.665
400	1.336	1.383	1.360	1.249
200	1.060	0.981	1.021	0.910
100	0.714	0.706	0.710	0.599
50	0.481	0.475	0.478	0.367
25	0.307	0.292	0.300	0.189
12.5	0.227	0.214	0.221	0.110
0	0.108	0.113	0.111

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

Time-course analysis of the effect of paraprobiotics ABG0050 on the intestinal immune system of broilers H Kondo, S Iino, T Fukuda, M Aoki, Y Yoshimura,Poultry Science,2025
Impact of multistrain probiotics on growth performance, immune response, and gut morphometry in broiler chicken Gallus gallus domesticus S Younas, DA Bukhari, Z Bibi, A Ullah, A Rehman,Poultry Science,2025
Effects of Escherichia coli strain Nissle 1917 on immune responses of Japanese quails (Coturnix japonica) to Newcastle disease vaccines R Abbasi Kali,Iranian Journal of Veterinary Research,2023
Ameliorative effects of Sida acuta and vitamin C on serum DNA damage, pro-inflammatory and anti-inflammatory cytokines in roosters fed aflatoxin B1 contaminated diets OJ Olarotimi,Olarotimi O J,,2023
Intranasal immunization with inactivated chlamydial elementary bodies formulated in VCG-chitosan nanoparticles induces robust immunity against intranasal Chlamydia psittaci challenge Zonghui Zuo,Scientific Reports,2021
Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,Parasitology Research,2021
Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,Animals,2019
Immunogenic potential and protective efficacy of a sptP deletion mutant of Salmonella Enteritidis as a live vaccine for chickens against a lethal challenge Yaxin Guo, et al,International Journal of Medical Microbiology,2019
The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,2019
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Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
Correlation between vascular endothelial growth factor and subclinical atherosclerosis in patients with psoriasis Shahidi-Dadras M. et al,Int J Dermatol.,2015
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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.