Chicken Interleukin 17,IL-17 ELISA Kit

產品詳情

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文獻引用 (6) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞白介素17(IL-17)酶聯免疫試劑盒（CSB-E04607Ch）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL17含量。IL-17是一種由Th17細胞分泌的促炎細胞因子，參與調節免疫反應和炎癥過程。其在銀屑病、類風濕關節炎等自身免疫疾病中起重要作用，同時也是腫瘤發生和進展的關鍵因子。研究IL-17機制有助于開發針對相關疾病的治療方法。試劑盒檢測范圍為150 pg/mL-6000 pg/mL，適用于家禽免疫學研究領域，如探究病原體感染后宿主免疫應答動態、評估疫苗免疫效果或解析炎癥性疾病模型中的細胞因子調控網絡，為禽類基礎免疫機制研究及疾病防控提供可靠工具。本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-17
Uniprot No.：

Q7T1P7
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

150 pg/mL-6000 pg/mL
靈敏度：

150 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	82-100
1:2	Average %	95
	Range %	90-100
1:4	Average %	96
	Range %	90-101
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of chicken IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	92	86-98

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.