Chicken Interleukin 12,IL-12/70 ELISA Kit

產品詳情

文獻引用 (2) 說明書下載

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

產品描述：

雞白介素12(IL-12/70)酶聯免疫試劑盒（CSB-E12836C）為競爭法ELISA試劑盒，定量檢測血清、血漿、組織勻漿樣本中的IL12/70含量。IL12/70作為靶點，IL-12和IL-70在免疫調節中發揮關鍵作用。針對它的研究聚焦于通過阻斷其信號通路，調節免疫細胞活性及功能來治療疾病，如自身免疫病、腫瘤等，旨在抑制異常免疫反應或增強抗腫瘤免疫。試劑盒檢測范圍為0.78 pg/mL-200 pg/mL，適用于家禽免疫機制研究、疫苗效力評估、病原感染模型中的細胞因子動態監測等科研場景，為禽類免疫相關疾病的分子機制探索提供可靠工具本品僅用于科研，不用于臨床診斷，產品具體參數及操作步驟詳見產品說明書。
縮寫：

IL-12/70
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測范圍：

0.78 pg/mL-200 pg/mL
靈敏度：

0.78 pg/mL
反應時間：

1-5h
樣本體積：

50-100ul
檢測波長：

450 nm
研究領域：

Immunology
測定原理：

quantitative
測定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IL-12/70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	88-98
1:2	Average %	94
	Range %	90-98
1:4	Average %	92
	Range %	87-98
1:8	Average %	96
	Range %	92-101

回收率：

The recovery of chicken IL-12/70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	92	88-97

標準曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.