Chicken Interferon γ,IFN-γ ELISA Kit

Chicken Interferon γ,IFN-γ ELISA Kit

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中文名稱：

雞γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒
貨號(hào)：

CSB-E08550Ch
規(guī)格：

96T/48T
價(jià)格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star? Award in the ELISA category

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產(chǎn)品描述：

雞γ干擾素(IFN-γ)酶聯(lián)免疫試劑盒（CSB-E08550Ch）為競(jìng)爭(zhēng)法ELISA試劑盒，定量檢測(cè)血清、血漿、組織勻漿樣本中的IFNG含量。干擾素-γ（IFNG）是一種II型干擾素，主要由自然殺傷細(xì)胞和活化的T細(xì)胞產(chǎn)生，具有抗病毒、免疫調(diào)節(jié)和抗腫瘤特性。其通過結(jié)合干擾素伽瑪受體（IFNGR）調(diào)節(jié)JAK-STAT通路，激活抗原提呈細(xì)胞，促進(jìn)I型輔助T細(xì)胞（Th1細(xì)胞）的分化，并抑制破骨細(xì)胞形成。研究機(jī)制涉及多種生物過程和細(xì)胞信號(hào)通路，如蛋白導(dǎo)入細(xì)胞核、轉(zhuǎn)錄調(diào)控、細(xì)胞凋亡和免疫反應(yīng)等。試劑盒檢測(cè)范圍為3.125 pg/mL-800 pg/mL，適用于家禽免疫機(jī)制研究、疫苗效力評(píng)估以及感染性疾病模型中免疫應(yīng)答動(dòng)態(tài)監(jiān)測(cè)，如禽流感、新城疫等病毒性疾病的免疫調(diào)控機(jī)制探索本品僅用于科研，不用于臨床診斷，產(chǎn)品具體參數(shù)及操作步驟詳見產(chǎn)品說明書。
別名：

IFNG ELISA Kit; Interferon gamma ELISA Kit; IFN-gamma ELISA Kit
縮寫：

IFNG
Uniprot No.：

P49708
種屬：

Gallus gallus (Chicken)
樣本類型：

serum, plasma, tissue homogenates
檢測(cè)范圍：

3.125 pg/mL-800 pg/mL
靈敏度：

3.125 pg/mL
反應(yīng)時(shí)間：

1-5h
樣本體積：

50-100ul
檢測(cè)波長(zhǎng)：

450 nm
研究領(lǐng)域：

Immunology
測(cè)定原理：

quantitative
測(cè)定方法：

Competitive

精密度：

線性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-γ in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	91-100
1:2	Average %	92
	Range %	88-97
1:4	Average %	102
	Range %	98-106
1:8	Average %	94
	Range %	90-98

回收率：

The recovery of chicken IFN-γ spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	93-101
EDTA plasma (n=4)	92	88-96

標(biāo)準(zhǔn)曲線：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average
800	0.178	0.182	0.180
200	0.245	0.233	0.239
50	0.457	0.482	0.470
12.5	0.848	0.817	0.833
3.125	1.396	1.364	1.380
0	2.237	2.242	2.240

數(shù)據(jù)處理：

ELISA操作視頻及標(biāo)曲繪制軟件下載
貨期：

3-5 working days

The inhibitory effect of Hypericum japonicum on H9N2 avian influenza virus H Hu, J Li, S Zheng, K Zhao, Y Xia, X Wei, M Han,Advanced Biotechnology,2024
Investigation of the Potential of Heterophil/Lymphocyte Ratio as a Biomarker to Predict Colonization Resistance and Inflammatory Response to Salmonella enteritidis Infection in Chicken M Thiam,Pathogens,2023
Effect of Two Particle Sizes of Nano Zinc Oxide on Growth Performance, Immune Function, Digestive Tract Morphology, and Intestinal Microbiota Composition in Broilers J Qu,Animals,2023
Impact of stocking density and dietary nano-zinc supplementation on stress indicators, immunity, and DNA damage in broiler chickens ? Sevim,Journal of the Hellenic Veterinary Medical Society,2023
Comparative Analysis of the Liver Transcriptome of Beijing You Chickens and Guang Ming Broilers under Salmonella enterica Serovar Typhimurium Infection Z Wang,Microorganisms,2022
Synergistic Immunosuppression of Avian Leukosis Virus Subgroup J and Infectious Bursal Disease Virus Is Responsible for Enhanced Pathogenicity W Chen,Viruses,2022
Evaluation of immunoprotective effects of recombinant protein and DNA vaccine based on Eimeria tenella surface antigen 16 and 22 in vivo Rui Fang,Parasitology Research,2021
Effects of different levels of Hermetia illucens larvae meal on performance, egg quality, yolk fatty acid composition and oxidative status of laying hens Xin Liu,Italian Journal of Animal Science,2021
In vivo immunoprotective comparison between recombinant protein and DNA vaccine of Eimeria tenella surface antigen 4 P Zhao,Veterinary Parasitology,2020
A Novel Adjuvant “Sublancin” Enhances Immune Response in Specific Pathogen-Free Broiler Chickens Inoculated with Newcastle Disease Vaccine Liu Y, et al,Journal of Immunology Research,2019
Effect of Acute Heat Stress on the mRNA Levels of Cytokines in Broiler Chickens Subjected to Embryonic Thermal Manipulation Saleh KMM, et al,Animals,2019
Immunogenic potential and protective efficacy of a sptP deletion mutant of Salmonella Enteritidis as a live vaccine for chickens against a lethal challenge Yaxin Guo, et al,International Journal of Medical Microbiology,2019
Lack of Toxic Interaction Between Fusariotoxins in Broiler Chickens Fed throughout Their Life at the Highest Level Tolerated in the European Union Metayer JP, et al,Toxins,2019
The effects and combinational effects of Bacillus subtilis and montmorillonite supplementation on performance, egg quality, oxidation status, and immune response in laying hens J.F.Chen, et al,The Egyptian Journal of Neurology, Psychiatry and Neurosurgery,2019
Effects of Supplementing Laying Hens with Purified Amino Acid Prepared from Animal Blood N. Joshi, et al,Tropical animal science journal,2019
Grape Seed Proanthocyanidin Extract Alleviates AflatoxinB1-Induced Immunotoxicity and Oxidative Stress via Modulation of NF-κB and Nrf2 Signaling Pathways in Broilers Shahid Ali Rajput, et al,Toxins,2019
Dietary Supplementation of Purified Amino Acid Derived from Animal Blood on Immune Response and Growth Performance of Broiler Chicken T. G. Wandita.et al,Tropical Animal Science Journal,2018
The molecular characterization and protective efficacy of microneme 3 of Eimeria mitis in chickens XinmeiHuang.et al,Veterinary Parasitology,2018
Gene cloning, expression and immune adjuvant properties of the recombinant fusion peptide Ta1-BLP on avian influenza inactivate virus vaccine CongZhang.et al,Microbial Pathogenesis,2018
Molecular characterisation and the protective immunity evaluation of Eimeria maxima surface antigen gene Tingqi Liu.et al,Parasites & Vectors,2018
Validation of immunomodulatory effects of lipopolysaccharide through expression profiling of Th1 and Th2 biased genes in Newcastle disease virus vaccinated indigenous chicken Rabia Bhardwaj.et al,Veterinary World,2018
Influenza Inactive Virus Vaccine with the Fusion Peptide (rTα1- BP5) Enhances Protection Against Influenza Through Humoral and Cell-Mediated Immunity Chen Wang.et al,,2016
Heat stress decreases expression of the cytokines, avian β-defensins 4 and 6 and Toll-like receptor 2 in broiler chickens infected with Salmonella Enteritidis W.M. Quinteiro-Filho.et al,Veterinary Immunology and Immunopathology,2017
Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses. Paudel S.et al,Virus Res.,2016
The Molecular Characterization and Immunity Identification of Microneme 3 of Eimeria acervulina. Zhang Z.et al,J Eukaryot Microbiol.,2016
Effect of Antibiotic Growth Promoters on Anti-oxidative and Anti-inflammatory Activities in Broiler Chickens Kabploy K.et al,The Thai Journal of Veterinary Medicine,2016
Immune responses to oral vaccination with Salmonella-delivered avian pathogenic Escherichia coli antigens and protective efficacy against colibacillosis. Lee JH.et al,Can J Vet Res.,2015
Omega-3 polyunsaturated fatty acids enrichment alters performance and immune response in infectious bursal disease challenged broilers Elham Maroufyan et al,Lipids in Health and Disease,2012
Improving the potency of DNA vaccine against Chicken Anemia Virus (CAV) by fusing VP1 protein of CAV to Marek's Disease Virus (MDV) Type-1 VP22 protein Hassan Moeini et al,Virology Journal,2011
Lactobacillus acidophilus as a live vehicle for oral immunization against chicken anemia virus Hassan Moeini et al,Tumor Biology,2010
Immune protection of Microneme 7 (EmMIC7) against Eimeria maxima challenge in chickens Huang J. et al,Avian Pathol,2015
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最新研究進(jìn)展：

IFNG，全稱為γ-干擾素，是一種由免疫細(xì)胞產(chǎn)生的細(xì)胞因子，在免疫反應(yīng)中起重要作用。最新研究發(fā)現(xiàn)，IFNG在免疫系統(tǒng)中調(diào)節(jié)T細(xì)胞、B細(xì)胞和自然殺傷細(xì)胞等免疫細(xì)胞的功能。IFNG還在感染和炎癥過程中發(fā)揮重要作用。研究表明，IFNG水平升高與多種疾病有關(guān)，包括自身免疫病、感染性疾病、腫瘤、心血管疾病和神經(jīng)退行性疾病等。近年來，研究人員正努力探索利用IFNG的治療作用，例如用IFNG治療乳腺癌、肺癌和黑色素瘤等。
功能：

Produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions. It is a potent activator of macrophages, it has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
基因功能參考文獻(xiàn)：
1. Knockdown of IFN-gamma did not seem to fully abrogate vaccine-induced protection against Marek's disease in chickens. PMID: 25673902
2. Vitiligo in Smyth line chickens appears to be a Th1-polarized autoimmune disease, whereby IFN-gamma expression is strongly associated with parallel increases in IL-10 and IL-21, particularly during the early and active stages of vitiligo. PMID: 22113479
3. The dose-response effects of supplemental dietary chormium on interferon-gamma expression in response to Newcastle disease virus vaccine in broiler chickens is reported. PMID: 17888472
4. The pivotal role of chicken interferon-gamma in the pathogenesis and immunosuppression of infectious bursal disease virus infection in in vivo, ex vivo and in vitro experiments are reported. PMID: 17899460
5. that in vitro stimulation with IBV resulted in ChIFN-gamma production in splenocytes of both infected birds and uninfected birds PMID: 19070879
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亞細(xì)胞定位：

Secreted.
蛋白家族：

Type II (or gamma) interferon family
數(shù)據(jù)庫鏈接：

KEGG: gga:396054

STRING: 9031.ENSGALP00000016086

UniGene: Gga.916

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.